The potential curative and hepatoprotective effects of platelet rich plasma on liver fibrosis in Schistosoma mansoni experimentally infected mice

• Published in: Journal of parasitic diseases 2023-06, Vol.47 (2), p.349-362
• Main Authors: Bayoumi, Angham M. A., Ismail, Mousa A. M., Mahmoud, Soheir S., Soliman, Ahmed S. A., Mousa, Amr M. A., Yousof, Hebat-Allah S. A.
• Format: Article
• Language: English
• Subjects: albino; Cirrhosis; Fibrosis; Granuloma; Granulomas; Health Promotion and Disease Prevention; histopathology; immunohistochemistry; Infections; Infectious Diseases; Liver; liver cirrhosis; Liver diseases; Medicine; Medicine & Public Health; Original Article; Platelets; Praziquantel; Schistosoma mansoni; Transforming growth factor-b1
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-023-01576-9

Trapped Schistosoma mansoni eggs trigger fibrotic liver disease that can continue to liver cirrhosis and failure. This work evaluates the outcome of platelet rich plasma (PRP) on S. mansoni -induced liver fibrosis by intraperitoneal (IP) and intrahepatic (IH) routes with/without Praziquantel (PZQ) treatment. Swiss albino mice ( n  = 162) were divided into non-infected ( n  = 66) and infected ( n  = 96) groups, then subdivided into non-treated and treated subgroups with PRP(IP), PRP(IH) 6th and 10th weeks post-infection, PZQ, PZQ + PRP(IP) and PZQ + PRP(IH) 6th and 10th weeks post-infection. Effects of treatments were evaluated by parasitological, histopathological and Immunohistochemical assessments. In the early assessment (12th week post-infection) of infected-treated groups, the mean granuloma number showed significant reduction in groups treated with PZQ + PRP (IH) 10th week, PRP (IP), PZQ + PRP (IP) and PZQ + PRP (IH) 6th week (33.33%, 33%, 27.77% and 27.22%, respectively). Furthermore, the mean granuloma diameter showed significant reduction in groups treated with PRP (IH) 10th week and PZQ + PRP (IP) (24.17% and 15.5%, respectively). Also, the fibrotic index showed significant reduction in groups treated with PZQ + PRP (IP), PRP (IP) and PZQ + PRP (IH) 6th week (48.18%, 46.81% and 41.36%, respectively). Transforming growth factor β1(TGF-β1) expression was in correlation with parasitological and histopathological results. Diminished TGF-β1 expression was mostly in infected groups treated with PZQ + PRP (IP), PZQ + PRP (IH) 6th week and PRP (IP) (88.63%, 88.63% and 77.27%, respectively). In the late assessment (14th week post-infection) of infected treated groups, TGF-β1expression was reduced in groups treated with PZQ, PRP (IH) 10th weeks, PRP (IP) (83.33%, 66.66%, 33.33% respectively). PRP showed promising anti-fibrotic effects on S. mansoni -induced liver fibrosis.