Antisense yycF and BMP-2 co-delivery gelatin methacryloyl and carboxymethyl chitosan hydrogel composite for infective bone defects regeneration

Repairing infected bone defects remains a challenge in clinical work. Intractable bacterial infections and insufficient osseointegration are major concerns for infected bone defects. To address these issues, we developed a gelatin methacryloyl (GelMA) and carboxymethyl chitosan (CMCS) composite hydr...

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Bibliographic Details
Published in:International journal of biological macromolecules 2023-12, Vol.253, p.127233-127233, Article 127233
Main Authors: Qin, Boquan, Dong, Hongxian, Tang, Xiaofang, Liu, Yunjie, Feng, Guoying, Wu, Shizhou, Zhang, Hui
Format: Article
Language:English
Subjects:
animal models
Antisense yycF
biocompatibility
biocompatible materials
bone formation
chitosan
femur
gelatin
hydrogels
Infective bone defects
Photo-crosslinked hydrogel
spring
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Summary:Repairing infected bone defects remains a challenge in clinical work. Intractable bacterial infections and insufficient osseointegration are major concerns for infected bone defects. To address these issues, we developed a gelatin methacryloyl (GelMA) and carboxymethyl chitosan (CMCS) composite hydrogel with BMP-2 growth factor and GO based antisense technology supported by a PLGA spring. In vitro, photo-crosslinked GelMA composite hydrogels shown excellent biocompatibility and degradability. Relying on the release of BMP-2 from the composite hydrogel provides osteogenic effects. The antisense yycF and BMP-2 were released with the degradation of GelMA and CMCS composite hydrogel. In terms of antimicrobial properties, CMCS, GO and post-transcriptional regulatory antisense yycF from the composite hydrogel synergistically kill S. aureus. In vivo, we implanted the composite hydrogel in a rat model of S. aureus infected femur defect, effectively accelerating bone healing in an infectious microenvironment. This research provides a novel biomaterial that is both antimicrobial and promotes bone regeneration, with the potential to treat infected bone defects.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.127233