Influence of photoactivation on tissue response to different dyes used in photodynamic therapy and laser ablation therapy

Laser ablation therapy (LA) uses Indocyanine Green dye (ICG) which efficiently absorbs laser energy and the increased temperature results in an instantaneous flame that chars tissue and microbes. Photodynamic therapy (PDT) uses different dyes that are activated by light to kill bacteria. This study...

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Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2024-02, Vol.251, p.112843-112843, Article 112843
Main Authors: Cintra, Luciano Tavares Angelo, Cantiga-Silva, Cristiane, Banci, Henrique Augusto, Faria, Flávio Duarte, da Silva Machado, Nathália Evelyn, Cardoso, Carolina de Barros Morais, de Oliveira, Pedro Henrique Chaves, Estrela, Lucas Rodrigues de Araújo, Sivieri-Araujo, Gustavo, Berbert, Fabio Luiz Camargo Villela, Garcia, Arturo Javier Aranda, Leonardo, Renato de Toledo
Format: Article
Language:English
Subjects:
analysis of variance
Biocompatibility
collagen
curcumin
Dyes
energy
histology
inflammation
Laser ablation
Laser activation
methylene blue
photobiology
photochemistry
photochemotherapy
Photodynamic therapy
polyethylene
rats
sodium chloride
temperature
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Summary:Laser ablation therapy (LA) uses Indocyanine Green dye (ICG) which efficiently absorbs laser energy and the increased temperature results in an instantaneous flame that chars tissue and microbes. Photodynamic therapy (PDT) uses different dyes that are activated by light to kill bacteria. This study evaluated the biocompatibility of the dye Curcumin (CUR), Methylene Blue (MB), and Indocyanine Green (ICG) before and after laser activation (ACT). Polyethylene tubes containing one of the dyes were implanted in the subcutaneous tissue of 32 rats (4 tubes per rat) which were divided into 8 groups: C - control (saline solution); C + ACT (Red Laser 660 nm); CUR; CUR + ACT (480 nm blue LED); MB; MB + ACT (Red Laser 660 nm); ICG; ICG + ACT (810 nm Infrared Laser). After 7 and 30 days (n = 8/time), the rats were euthanized and the tubes with the surrounding tissue were removed and processed for histological analysis of inflammation using H&E stain, and collagen fiber maturation using picrosirius red (PSR). A two-way analysis of variance statistical test was applied (p 
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2024.112843