High starch diets attenuate the immune function of Micropterus salmoides immune organs by modulating Keap1/Nrf2 and MAPK signaling pathways

• Published in: Fish & shellfish immunology 2023-11, Vol.142, p.109079-109079, Article 109079
• Main Authors: Liu, Hongli, Chen, Baipeng, Cao, Yanhao, Geng, Yi, Ouyang, Ping, Chen, Defang, Li, Liangyu, Huang, Xiaoli
• Format: Article
• Language: English
• Subjects: antioxidant activity; disease prevention; enzyme activity; fish; fish feeds; gene expression; high carbohydrate diet; High starch; Immune organs; immune response; Inflammation; kidneys; lysozyme; Micropterus salmoides; Oxidative stress; shellfish; spleen; starch
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2023.109079

Based on their good physiological functions and physical properties, carbohydrates are widely used in fish feed. However, excessive use of carbohydrates such as starch in fish feed may reduce the immunity of the fish and cause a series of health problems. In order to more clearly clarify the effects of different starch levels in feed on the immune organs of Micropterus salmoides, this study took the immune organs as the entry point and explored it from several perspectives, including differences in enzyme activity in plasma, changes in gene expression in immune organs, and resistance to pathogenic bacteria. The results showed that (1) high starch feed activates inflammatory responses in the spleen and head kidney through the MAPK signaling pathway. This leads to a decrease in the number of lymphocytes and weakens the resistance to pathogens; (2) high starch diet affects the antioxidant capacity of the trunk kidney by regulating the Keap1/Nrf2 pathway; (3) There was a strong correlation between gene expression patterns in the head kidney and lysozyme content in plasma. This implies that the high starch diet may regulate lysozyme production by affecting gene expression in the head kidney and further affect immune function. This study helps to reveal the interaction between starch and the immune system and provide scientific basis for the development of reasonable dietary recommendations and disease prevention. •High starch feed activates inflammatory responses in the spleen and head kidney through the MAPK signaling pathway.•High starch diet affects the antioxidant capacity of the trunk kidney by regulating the Keap1/Nrf2 pathway.•High starch diet may regulate lysozyme production through gene expression in the head kidney, affecting immune function.