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Mycorrhizal symbiosis alleviates Mn toxicity and downregulates Mn transporter genes in Eucalyptus tereticornis under contrasting soil phosphorus
Background and aims Tropical soils often present two issues that can hinder plant growth: low phosphorus (P) and high manganese (Mn) availability. Eucalyptus tereticornis is frequently cultivated in such soils. We investigated the effects of Mn in E. tereticornis under contrasting soil P availabilit...
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| Published in: | Plant and soil 2023-08, Vol.489 (1-2), p.361-383 |
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| creator | De Oliveira, Vinicius H. Montanha, Gabriel S. Carvalho, Hudson W. P. Mazzafera, Paulo de Andrade, Sara Adrián L. |
| description | Background and aims
Tropical soils often present two issues that can hinder plant growth: low phosphorus (P) and high manganese (Mn) availability.
Eucalyptus tereticornis
is frequently cultivated in such soils. We investigated the effects of Mn in
E. tereticornis
under contrasting soil P availability and hypothesized that arbuscular mycorrhizal (AM) symbiosis could alleviate Mn toxicity by improving P nutrition and altering the expression of Mn transporter genes.
Methods
Inoculated (AM) and non-inoculated (NM) seedlings grew for seven months in a soil with low or sufficient P availability, under three Mn doses: control, 75 and 150 mg kg
−1
Mn. We assessed growth, AM colonization, nutrient concentrations, and the expression of eight genes related to Mn transport and homeostasis in roots. Mn distribution at one-leaf level was determined by μ-XRF.
Results
Low P exacerbated Mn toxicity and hindered plant growth. Mycorrhizal symbiosis did not influence Mn accumulation, but improved growth and Mn tolerance at low P, partly by improving P nutrition. At sufficient P, foliar Mn reached 3500 mg kg
−1
, and μ-XRF patterns suggest preferential accumulation in the leaf lamina compared to margins or midribs. In NM plants, the vacuolar transporters
EtVIT1
and
EtMTP8
and the Mn-nicotianamine influx transporter
EtYSL6
were the most responsive genes to Mn, while in AM roots most were downregulated.
Conclusion
Vacuolar sequestration and transport of complexed Mn are important mechanisms behind Mn tolerance in
E. tereticornis
. We propose that Mn is transported via the mycorrhizal pathway, explaining why it does not elicit the same molecular response observed in NM roots. |
| doi_str_mv | 10.1007/s11104-023-06024-4 |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153174480</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A763273425</galeid><sourcerecordid>A763273425</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-84baf2a382c43f45968cee2a0e2dab93272bcc3c619aa88842d373b03092cbd23</originalsourceid><addsrcrecordid>eNp9UcuKFTEUDKLgdfQHXAXcuOmZvPq1HIbxATO4UXAX0ul0T4bcpM1Jq-1X-Mmeaw8KLiSEkErVOXVShLzk7Jwz1l4A55ypiglZsYYJValH5MDrVlY1k81jcmBMioq1_een5BnAPTvdeXMgP283m3K-8z9MoLAdB5_AAzUhuK_eFAf0NtKSvnvry0ZNHOmYvsXs5jX8ec0mwpJycZnOLiLoI71erQnbUlagiLvisUvEwmsckWZTRBUUH2cKyQe63CXAnVd4Tp5MJoB78XCekU9vrj9evatuPrx9f3V5U1nZ81J1ajCTMLITVslJ1X3TWeeEYU6MZuilaMVgrbQN743puk6JUbZyYJL1wg6jkGfk9V53yenL6qDoowfrQjDRpRW05LXkrVIdQ-qrf6j3ac0R3WnR4W8zbFIj63xnzSY47eOUcESLa3RHnD66ySN-2TboTSpxEohdYHMCyG7SS_ZHkzfNmT6lqvdUNaaqf6eqFYrkLgIkx9nlv17-o_oFwf2o0Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2860206195</pqid></control><display><type>article</type><title>Mycorrhizal symbiosis alleviates Mn toxicity and downregulates Mn transporter genes in Eucalyptus tereticornis under contrasting soil phosphorus</title><source>Springer Link</source><creator>De Oliveira, Vinicius H. ; Montanha, Gabriel S. ; Carvalho, Hudson W. P. ; Mazzafera, Paulo ; de Andrade, Sara Adrián L.</creator><creatorcontrib>De Oliveira, Vinicius H. ; Montanha, Gabriel S. ; Carvalho, Hudson W. P. ; Mazzafera, Paulo ; de Andrade, Sara Adrián L.</creatorcontrib><description>Background and aims
Tropical soils often present two issues that can hinder plant growth: low phosphorus (P) and high manganese (Mn) availability.
Eucalyptus tereticornis
is frequently cultivated in such soils. We investigated the effects of Mn in
E. tereticornis
under contrasting soil P availability and hypothesized that arbuscular mycorrhizal (AM) symbiosis could alleviate Mn toxicity by improving P nutrition and altering the expression of Mn transporter genes.
Methods
Inoculated (AM) and non-inoculated (NM) seedlings grew for seven months in a soil with low or sufficient P availability, under three Mn doses: control, 75 and 150 mg kg
−1
Mn. We assessed growth, AM colonization, nutrient concentrations, and the expression of eight genes related to Mn transport and homeostasis in roots. Mn distribution at one-leaf level was determined by μ-XRF.
Results
Low P exacerbated Mn toxicity and hindered plant growth. Mycorrhizal symbiosis did not influence Mn accumulation, but improved growth and Mn tolerance at low P, partly by improving P nutrition. At sufficient P, foliar Mn reached 3500 mg kg
−1
, and μ-XRF patterns suggest preferential accumulation in the leaf lamina compared to margins or midribs. In NM plants, the vacuolar transporters
EtVIT1
and
EtMTP8
and the Mn-nicotianamine influx transporter
EtYSL6
were the most responsive genes to Mn, while in AM roots most were downregulated.
Conclusion
Vacuolar sequestration and transport of complexed Mn are important mechanisms behind Mn tolerance in
E. tereticornis
. We propose that Mn is transported via the mycorrhizal pathway, explaining why it does not elicit the same molecular response observed in NM roots.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/s11104-023-06024-4</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Accumulation ; Agriculture ; Analysis ; Arbuscular mycorrhizas ; Availability ; Biomedical and Life Sciences ; Down-regulation ; Ecology ; Eucalyptus ; Eucalyptus tereticornis ; Gene expression ; Genes ; Growth ; Homeostasis ; Leaves ; Life Sciences ; Manganese ; Nicotianamine ; Nutrient concentrations ; Nutrient transport ; nutrition ; Phosphorus ; Phosphorus content ; Plant growth ; Plant Physiology ; Plant Sciences ; Research Article ; Roots ; Seedlings ; soil ; Soil investigations ; Soil Science & Conservation ; Soils ; Symbiosis ; Toxicity ; Tropical environments ; Tropical soils ; vacuoles ; vesicular arbuscular mycorrhizae</subject><ispartof>Plant and soil, 2023-08, Vol.489 (1-2), p.361-383</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>COPYRIGHT 2023 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-84baf2a382c43f45968cee2a0e2dab93272bcc3c619aa88842d373b03092cbd23</citedby><cites>FETCH-LOGICAL-c391t-84baf2a382c43f45968cee2a0e2dab93272bcc3c619aa88842d373b03092cbd23</cites><orcidid>0000-0002-6066-5827</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>De Oliveira, Vinicius H.</creatorcontrib><creatorcontrib>Montanha, Gabriel S.</creatorcontrib><creatorcontrib>Carvalho, Hudson W. P.</creatorcontrib><creatorcontrib>Mazzafera, Paulo</creatorcontrib><creatorcontrib>de Andrade, Sara Adrián L.</creatorcontrib><title>Mycorrhizal symbiosis alleviates Mn toxicity and downregulates Mn transporter genes in Eucalyptus tereticornis under contrasting soil phosphorus</title><title>Plant and soil</title><addtitle>Plant Soil</addtitle><description>Background and aims
Tropical soils often present two issues that can hinder plant growth: low phosphorus (P) and high manganese (Mn) availability.
Eucalyptus tereticornis
is frequently cultivated in such soils. We investigated the effects of Mn in
E. tereticornis
under contrasting soil P availability and hypothesized that arbuscular mycorrhizal (AM) symbiosis could alleviate Mn toxicity by improving P nutrition and altering the expression of Mn transporter genes.
Methods
Inoculated (AM) and non-inoculated (NM) seedlings grew for seven months in a soil with low or sufficient P availability, under three Mn doses: control, 75 and 150 mg kg
−1
Mn. We assessed growth, AM colonization, nutrient concentrations, and the expression of eight genes related to Mn transport and homeostasis in roots. Mn distribution at one-leaf level was determined by μ-XRF.
Results
Low P exacerbated Mn toxicity and hindered plant growth. Mycorrhizal symbiosis did not influence Mn accumulation, but improved growth and Mn tolerance at low P, partly by improving P nutrition. At sufficient P, foliar Mn reached 3500 mg kg
−1
, and μ-XRF patterns suggest preferential accumulation in the leaf lamina compared to margins or midribs. In NM plants, the vacuolar transporters
EtVIT1
and
EtMTP8
and the Mn-nicotianamine influx transporter
EtYSL6
were the most responsive genes to Mn, while in AM roots most were downregulated.
Conclusion
Vacuolar sequestration and transport of complexed Mn are important mechanisms behind Mn tolerance in
E. tereticornis
. We propose that Mn is transported via the mycorrhizal pathway, explaining why it does not elicit the same molecular response observed in NM roots.</description><subject>Accumulation</subject><subject>Agriculture</subject><subject>Analysis</subject><subject>Arbuscular mycorrhizas</subject><subject>Availability</subject><subject>Biomedical and Life Sciences</subject><subject>Down-regulation</subject><subject>Ecology</subject><subject>Eucalyptus</subject><subject>Eucalyptus tereticornis</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Growth</subject><subject>Homeostasis</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Manganese</subject><subject>Nicotianamine</subject><subject>Nutrient concentrations</subject><subject>Nutrient transport</subject><subject>nutrition</subject><subject>Phosphorus</subject><subject>Phosphorus content</subject><subject>Plant growth</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Research Article</subject><subject>Roots</subject><subject>Seedlings</subject><subject>soil</subject><subject>Soil investigations</subject><subject>Soil Science & Conservation</subject><subject>Soils</subject><subject>Symbiosis</subject><subject>Toxicity</subject><subject>Tropical environments</subject><subject>Tropical soils</subject><subject>vacuoles</subject><subject>vesicular arbuscular mycorrhizae</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9UcuKFTEUDKLgdfQHXAXcuOmZvPq1HIbxATO4UXAX0ul0T4bcpM1Jq-1X-Mmeaw8KLiSEkErVOXVShLzk7Jwz1l4A55ypiglZsYYJValH5MDrVlY1k81jcmBMioq1_een5BnAPTvdeXMgP283m3K-8z9MoLAdB5_AAzUhuK_eFAf0NtKSvnvry0ZNHOmYvsXs5jX8ec0mwpJycZnOLiLoI71erQnbUlagiLvisUvEwmsckWZTRBUUH2cKyQe63CXAnVd4Tp5MJoB78XCekU9vrj9evatuPrx9f3V5U1nZ81J1ajCTMLITVslJ1X3TWeeEYU6MZuilaMVgrbQN743puk6JUbZyYJL1wg6jkGfk9V53yenL6qDoowfrQjDRpRW05LXkrVIdQ-qrf6j3ac0R3WnR4W8zbFIj63xnzSY47eOUcESLa3RHnD66ySN-2TboTSpxEohdYHMCyG7SS_ZHkzfNmT6lqvdUNaaqf6eqFYrkLgIkx9nlv17-o_oFwf2o0Q</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>De Oliveira, Vinicius H.</creator><creator>Montanha, Gabriel S.</creator><creator>Carvalho, Hudson W. P.</creator><creator>Mazzafera, Paulo</creator><creator>de Andrade, Sara Adrián L.</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7X2</scope><scope>88A</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>SOI</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-6066-5827</orcidid></search><sort><creationdate>20230801</creationdate><title>Mycorrhizal symbiosis alleviates Mn toxicity and downregulates Mn transporter genes in Eucalyptus tereticornis under contrasting soil phosphorus</title><author>De Oliveira, Vinicius H. ; Montanha, Gabriel S. ; Carvalho, Hudson W. P. ; Mazzafera, Paulo ; de Andrade, Sara Adrián L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-84baf2a382c43f45968cee2a0e2dab93272bcc3c619aa88842d373b03092cbd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Accumulation</topic><topic>Agriculture</topic><topic>Analysis</topic><topic>Arbuscular mycorrhizas</topic><topic>Availability</topic><topic>Biomedical and Life Sciences</topic><topic>Down-regulation</topic><topic>Ecology</topic><topic>Eucalyptus</topic><topic>Eucalyptus tereticornis</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Growth</topic><topic>Homeostasis</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Manganese</topic><topic>Nicotianamine</topic><topic>Nutrient concentrations</topic><topic>Nutrient transport</topic><topic>nutrition</topic><topic>Phosphorus</topic><topic>Phosphorus content</topic><topic>Plant growth</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Research Article</topic><topic>Roots</topic><topic>Seedlings</topic><topic>soil</topic><topic>Soil investigations</topic><topic>Soil Science & Conservation</topic><topic>Soils</topic><topic>Symbiosis</topic><topic>Toxicity</topic><topic>Tropical environments</topic><topic>Tropical soils</topic><topic>vacuoles</topic><topic>vesicular arbuscular mycorrhizae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Oliveira, Vinicius H.</creatorcontrib><creatorcontrib>Montanha, Gabriel S.</creatorcontrib><creatorcontrib>Carvalho, Hudson W. P.</creatorcontrib><creatorcontrib>Mazzafera, Paulo</creatorcontrib><creatorcontrib>de Andrade, Sara Adrián L.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Oliveira, Vinicius H.</au><au>Montanha, Gabriel S.</au><au>Carvalho, Hudson W. P.</au><au>Mazzafera, Paulo</au><au>de Andrade, Sara Adrián L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycorrhizal symbiosis alleviates Mn toxicity and downregulates Mn transporter genes in Eucalyptus tereticornis under contrasting soil phosphorus</atitle><jtitle>Plant and soil</jtitle><stitle>Plant Soil</stitle><date>2023-08-01</date><risdate>2023</risdate><volume>489</volume><issue>1-2</issue><spage>361</spage><epage>383</epage><pages>361-383</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><abstract>Background and aims
Tropical soils often present two issues that can hinder plant growth: low phosphorus (P) and high manganese (Mn) availability.
Eucalyptus tereticornis
is frequently cultivated in such soils. We investigated the effects of Mn in
E. tereticornis
under contrasting soil P availability and hypothesized that arbuscular mycorrhizal (AM) symbiosis could alleviate Mn toxicity by improving P nutrition and altering the expression of Mn transporter genes.
Methods
Inoculated (AM) and non-inoculated (NM) seedlings grew for seven months in a soil with low or sufficient P availability, under three Mn doses: control, 75 and 150 mg kg
−1
Mn. We assessed growth, AM colonization, nutrient concentrations, and the expression of eight genes related to Mn transport and homeostasis in roots. Mn distribution at one-leaf level was determined by μ-XRF.
Results
Low P exacerbated Mn toxicity and hindered plant growth. Mycorrhizal symbiosis did not influence Mn accumulation, but improved growth and Mn tolerance at low P, partly by improving P nutrition. At sufficient P, foliar Mn reached 3500 mg kg
−1
, and μ-XRF patterns suggest preferential accumulation in the leaf lamina compared to margins or midribs. In NM plants, the vacuolar transporters
EtVIT1
and
EtMTP8
and the Mn-nicotianamine influx transporter
EtYSL6
were the most responsive genes to Mn, while in AM roots most were downregulated.
Conclusion
Vacuolar sequestration and transport of complexed Mn are important mechanisms behind Mn tolerance in
E. tereticornis
. We propose that Mn is transported via the mycorrhizal pathway, explaining why it does not elicit the same molecular response observed in NM roots.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s11104-023-06024-4</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0002-6066-5827</orcidid></addata></record> |
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| ispartof | Plant and soil, 2023-08, Vol.489 (1-2), p.361-383 |
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| subjects | Accumulation Agriculture Analysis Arbuscular mycorrhizas Availability Biomedical and Life Sciences Down-regulation Ecology Eucalyptus Eucalyptus tereticornis Gene expression Genes Growth Homeostasis Leaves Life Sciences Manganese Nicotianamine Nutrient concentrations Nutrient transport nutrition Phosphorus Phosphorus content Plant growth Plant Physiology Plant Sciences Research Article Roots Seedlings soil Soil investigations Soil Science & Conservation Soils Symbiosis Toxicity Tropical environments Tropical soils vacuoles vesicular arbuscular mycorrhizae |
| title | Mycorrhizal symbiosis alleviates Mn toxicity and downregulates Mn transporter genes in Eucalyptus tereticornis under contrasting soil phosphorus |
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