A study into the diversity of coral-associated bacteria using culture-dependent and culture-independent approaches in coral Dipsastraea favus from the Gulf of Kutch

• Published in: Marine pollution bulletin 2024-04, Vol.201, p.116172-116172, Article 116172
• Main Authors: Patel, Zarna Z., Joshi, Himanshu, Puvar, Apurvasinh, Pandit, Ramesh, Joshi, Chaitanya, Joshi, Madhvi, Tipre, Devayani R.
• Format: Article
• Language: English
• Subjects: 16S rRNA gene; Antibiotic resistance; biodiversity; coral reefs; Coral-associated bacteria (CAB); corals; Culture-dependent; Culture-independent; ecosystems; Exiguobacterium; India; marine pollution; Metagenomic; metagenomics; multiple drug resistance; Pseudoalteromonas; Shewanella; Streptomyces; Vibrio
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2024.116172

Corals harbour ~25 % of the marine diversity referring to biodiversity hotspots in marine ecosystems. Global efforts to find ways to restore the coral reef ecosystem from various threats can be complemented by studying coral-associated bacteria. Coral-associated bacteria are vital components of overall coral wellbeing. We explored the bacterial diversity associated with coral Dipsastraea favus (D. favus) collected from the Gulf of Kutch, India, using both culture-dependent and metagenomic approaches. In both approaches, phylum Proteobacteria, Firmicutes, and Actinobacteria predominated, comprising the genera Vibrio, Bacillus, Shewanella, Pseudoalteromonas, Exiguobacterium and Streptomyces. Moreover, the majority of culturable isolates showed multiple antibiotic resistance index ≥0.2. In this study, specific bacterial diversity associated with coral sp. D. favus and its possible role in managing coral health was established. Almost 43 strains from the samples were successfully cultured, creating a base for exploring these microbes for their potential use in coral conservation methods. •Combined approach culture-dependent and independent for coral bacterial diversity -1st report.•Report on dominant genera among both approaches and their potential role in the coral ecosystem.•Culturable isolates tested against 20 antibiotics for their antibiotic resistance profile.•Antibiotic resistance profiles show insight into the bacteria as a strategy for improving fitness under stress.•Role of beneficial cultivable bacteria as probiotics might help in coral conservation.