Comprehensive investigation of BZR gene family in four dicots and the function of PtBZR9 and PtBZR12 under drought stress

• Published in: Plant physiology and biochemistry 2024-02, Vol.207, p.108360-108360, Article 108360
• Main Authors: Wang, Linna, Lin, Miao, Zou, Lina, Zhang, Shunran, Lan, Yangang, Yan, Hanwei, Xiang, Yan
• Format: Article
• Language: English
• Subjects: Arabidopsis thaliana; brassinosteroids; BZR; Carica papaya; Drought stress; family; genes; genetically modified organisms; phenotype; plant growth; Poplar; Populus trichocarpa; PtBZR12; PtBZR9; signal transduction; stress tolerance; transcription factors; Vitis vinifera; water stress
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108360

Brassinazole-resistant (BZR) transcription factor plays an important role in plant growth and stress resistance through brassinosteroid (BR) signal transduction. However, systematic analysis of the BZR family in dicots remains limited. In this study, we conducted a genome-wide study of four typical dicots: Arabidopsis thaliana, Carica papaya, Vitis vinifera and Populus trichocarpa. Thirty-four BZR gene family members were identified and classified them into three subfamilies. Analysis of promoter and expression patterns revealed crucial role of a pair of homologous BZR genes, PtBZR9 and PtBZR12, in poplar may play a critical role under abiotic stress. PtBZR9 and PtBZR12 were localised in the nucleus and exhibited mutual interactions. Moreover, transient overexpression (OE) of PtBZR9 and PtBZR12 in poplar enhanced tolerance to drought stress. The phenotypic and physiological characteristics of PtBZR9 and PtBZR12 OE in Arabidopsis mirrored those of transient OE in the poplar. Additionally, PtBZR9 and PtBZR12 can bind to the E-box element. Under exogenous BR treatment, transgenic lines displayed a greater decrease in root length than the wild type. Thus, these findings provide a solid foundation for future research on the complex regulatory mechanisms of BZR genes. •Thirty-four BZR genes were identified, revealing a highly conserved and structurally similar evolution of BZR family.•Homologous genes PtBZR9 and PtBZR12 exhibited mutual interaction and binding to the E-box elements.•Transgenic plants of PtBZR9 and PtBZR12 plants exhibited strong drought tolerance.•PtBZR9 and PtBZR12 may be involved in the BR signaling pathway for regulate drought stress.