Assessment of oxidative stress biomarkers in the threatened annual killifish Austrolebias charrua exposed to Roundup

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2024-02, Vol.276, p.109787-109787, Article 109787
• Main Authors: Pagano, Antônio Duarte, Gonçalves, Natiéli Machado, Domingues, William Borges, da Silveira, Tony Leandro Rezende, Kütter, Mateus Tavares, Junior, Antônio Sérgio Varela, Corcini, Carine Dahl, Nascimento, Mariana Cavalcanti, dos Reis, Luana Ferreira Viana, Costa, Patrícia Gomes, Bianchini, Adalto, Volcan, Matheus Vieira, Remião, Mariana Härter, Campos, Vinicius Farias
• Format: Article
• Language: English
• Subjects: acute exposure; antioxidant activity; Austrolebias charrua; biomarkers; brain; DNA damage; Ecotoxicology; erythrocytes; Extinction; fish; Flow cytometry; Gene expression; glyphosate; lipid peroxidation; liver; membrane fluidity; Neotropics; oxidative stress; Pesticide; physiology; toxicity; Wildlife
• ISSN: 1532-0456
• DOI: 10.1016/j.cbpc.2023.109787

This study aimed to analyze the toxic effects of Roundup Transorb® on the endangered Neotropical annual killifish Austrolebias charrua through the assessment of molecular and biochemical biomarkers. The fish were collected in temporary ponds and exposed to environmentally realistic concentrations of the herbicide (5 mg.L‐1 for 96 h). The production of ROS, lipid peroxidation, DNA damage, and membrane fluidity were evaluated in the blood cells by flow cytometry. The mRNA expression of the antioxidant-related genes sod2, cat, gstα, atp1a1, gclc, and ucp1 across the brain, liver, and gills was quantified. The acute exposure of annual killifish to Roundup significantly increased ROS production, lipid peroxidation, and DNA damage in their erythrocytes. Likewise, Roundup Transorb® decreased membrane fluidity in the blood cells of the exposed fish. Gene expression analysis revealed that Roundup exposure alters the relative expression of genes associated with oxidative stress and antioxidant defense. Our results give rise to new insights into adaptive mechanisms of A. charrua in response to Roundup. Since Brazilian annual killifishes strongly risk extinction, this study paves the way for developing novel biotechnologies applied to environmental monitoring and aquatic toxicology assessment. [Display omitted] •Pesticide pollution drives the extinction of Brazilian annual killifishes•Roundup increases erythrocytes ROS production, LPO, and DNA damage in A. charrua•Exposure to Roundup modulates the expression of antioxidant genes in A. charrua•Flow cytometry and qPCR are reliable tools to assess water pollution by herbicides•Exposure to Roundup induces systemic oxidative stress in annual killifish