Degradation of polypropylene by fungi Coniochaeta hoffmannii and Pleurostoma richardsiae

• Published in: Microbiological research 2023-12, Vol.277, p.127507-127507, Article 127507
• Main Authors: Porter, Rachel, Černoša, Anja, Fernández-Sanmartín, Paola, Cortizas, Antonio Martínez, Aranda, Elisabet, Luo, Yonglun, Zalar, Polona, Podlogar, Matejka, Gunde-Cimerman, Nina, Gostinčar, Cene
• Format: Article
• Language: English
• Subjects: Biodegradation; carbohydrates; codon usage; Coniochaeta; electron microscopy; fabrics; Fourier transform infrared spectroscopy; Fungi; Hydrocarbons; Plastic; plastics; Pleurostoma; polyethylene terephthalates; polypropylenes; polyurethanes; proteome; Raman spectroscopy; reflectance; Spectroscopy; transcriptome
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2023.127507

The urgent need for better disposal and recycling of plastics has motivated a search for microbes with the ability to degrade synthetic polymers. While microbes capable of metabolizing polyurethane and polyethylene terephthalate have been discovered and even leveraged in enzymatic recycling approaches, microbial degradation of additive-free polypropylene (PP) remains elusive. Here we report the isolation and characterization of two fungal strains with the potential to degrade pure PP. Twenty-seven fungal strains, many isolated from hydrocarbon contaminated sites, were screened for degradation of commercially used textile plastic. Of the candidate strains, two identified as Coniochaeta hoffmannii and Pleurostoma richardsiae were found to colonize the plastic fibers using scanning electron microscopy (SEM). Further experiments probing degradation of pure PP films were performed using C. hoffmannii and P. richardsiae and analyzed using SEM, Raman spectroscopy and Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR). The results showed that the selected fungi were active against pure PP, with distinct differences in the bonds targeted and the degree to which each was altered. Whole genome and transcriptome sequencing was conducted for both strains and the abundance of carbohydrate active enzymes, GC content, and codon usage bias were analyzed in predicted proteomes for each. Enzymatic assays were conducted to assess each strain’s ability to degrade naturally occurring compounds as well as synthetic polymers. These investigations revealed potential adaptations to hydrocarbon-rich environments and provide a foundation for further investigation of PP degrading activity in C. hoffmannii and P. richardsiae. [Display omitted] •Fungi Pleurostoma richardsiae and Coniochaeta hoffmannii grow on polypropylene.•Both fungi transform polypropylene, targeting different chemical bonds.•Both fungi produce a wide spectrum of extracellular enzymes.•Hydrocarbon-contaminated environment may enrich for plastic degraders.