miR-199a-3p suppresses neuroinflammation by directly targeting MyD88 in a mouse model of bone cancer pain

• Published in: Life sciences (1973) 2023-11, Vol.333, p.122139-122139, Article 122139
• Main Authors: Saadh, Mohamed J., Rashed, Amera Bekhatroh, Jamal, Azfar, Castillo-Acobo, Roxana Yolanda, Kamal, Mohammad Azhar, Cotrina-Aliaga, Juan Carlos, Gonzáles, José Luis Arias, Alothaim, Abdulaziz S., Alhoqail, Wardah A., Ahmad, Fuzail, Lakshmaiya, Natrayan, Amin, Ali H., Younus, Dhuha Ghassan, Rojas, Gregorio Gilmer Rosales, Bahrami, Abolfazl, Akhavan-Sigari, Reza
• Format: Article
• Language: English
• Subjects: agonists; animal models; Astrocyte activation; astrocytes; Bone cancer pain; cytokines; femur; loss-of-function mutation; luciferase; mice; microRNA; miR-199a-3p; MyD88; neoplasm cells; Neuroinflammation; NF-κB signaling pathway; pain; quality of life; sequence analysis; spinal cord; therapeutics
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2023.122139

Pain is a profoundly debilitating symptom in cancer patients, leading to disability, immobility, and a marked decline in their quality of life. This study aimed to investigate the potential roles of miR-199a-3p in a murine model of bone cancer pain induced by tumor cell implantation in the medullary cavity of the femur. We assessed pain-related behaviors, including the paw withdrawal mechanical threshold (PWMT) and the number of spontaneous flinches (NSF). To investigate miRNA expression and its targets in astrocytes, we employed a combination of RNA-seq analysis, qRT-PCR, Western blotting, EdU, TUNEL, ChIP, ELISA, and luciferase reporter assays in mice (C3H/HeJ) with bone cancer pain and control groups. On days 10, 14, 21, and 28 post-surgery, we observed significant differences in PWTL, PWMT, and NSF when compared to the sham group (P