Co-immobilized multi-enzyme biocatalytic system on reversible and soluble carrier for saccharification of corn straw cellulose

• Published in: Bioresource technology 2024-03, Vol.395, p.130325-130325, Article 130325
• Main Authors: Yu, Xiaoxiao, Li, Jianzhen, Sun, Yan, Xie, Yubing, Su, Yingjie, Tang, Shanshan, Bian, Sijia, Liu, Liying, Huo, Fei, Huang, Qing, Chen, Guang
• Format: Article
• Language: English
• Subjects: cellulose; Co-immobilization enzyme; corn straw; endo-1,4-beta-glucanase; enzyme activity; glucose; Lignocellulose; polygalacturonase; Saccharification; solubility; Synergistic effect
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2024.130325

[Display omitted] •Saccharification of cellulose to glucose was achieved on immobilized enzymes.•Tri-enzyme biocatalyst of cellulase, β-glucosidase and pectinase was prepared.•The glucose was obtained from cellulose with conversion rate of 74.45%. Herein, three enzymes (cellulase, β-glucosidase, and pectinase) with synergistic effects were co-immobilized on the Eudragit L-100, and the recovery of co-immobilized enzymes from solid substrates were achieved through the reversible and soluble property of the carrier. The optimization of enzyme ratio overcomed the problem of inappropriate enzyme activity ratio caused by different immobilization efficiencies among enzymes during the preparation process of co-immobilized enzymes. The co-immobilized enzymes were utilized to catalytically hydrolyze cellulose from corn straw into glucose, achieving a cellulose conversion rate of 74.45% under conditions optimized for their enzymatic characteristics and hydrolytic reaction conditions. As a result of the reversibility and solubility of the carrier, the co-immobilized enzymes were recovered from the solid substrate after five cycles, retaining 54.67% of the enzyme activity. The aim of this study is to investigate the potential of co-immobilizing multiple enzymes onto the Eudragit L-100 carrier for the synergistic degradation of straw cellulose.