Geometry of posterior larval spiracles to identify medically and forensically important calliphorids in Thailand

•The first report of landmarks-based approach on posterior spiracles of blow fly larvae.•High percentage of correct classification in most species, ranking from 86% to 100%.•Our data (800 specimens) provide as a data bank for further analysis. Fly identification is the primary step of analysis in fo...

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Bibliographic Details
Published in:Acta tropica 2024-04, Vol.252, p.107126-107126, Article 107126
Main Authors: Kanta, Wanida, Limsopatham, Kwankamol, Sukontason, Kabkaew L., Sukontason, Kom, Dujardin, Jean-Pierre, Dujardin, Sebastien, Sanit, Sangob
Format: Article
Language:English
Subjects:
Chrysomya megacephala
Forensic entomology
forensic sciences
Geometric morphometrics
geometry
humans
Identification
larvae
Lucilia cuprina
morphometry
Posterior spiracles
species
species identification
spiracles
Thailand
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Summary:•The first report of landmarks-based approach on posterior spiracles of blow fly larvae.•High percentage of correct classification in most species, ranking from 86% to 100%.•Our data (800 specimens) provide as a data bank for further analysis. Fly identification is the primary step of analysis in forensic entomology. Although morphology and molecular techniques are considered satisfactory methods, some constraints may arise from a financial or even human point of view. Over the past decade, the geometric morphometric approach has been increasingly advocated for the classification and identification of arthropods. This study explored the method for species identification of 800 third-instar larvae of eight blow fly species of medical and forensic importance: Chrysomya chani Kurahashi, Chrysomya megacephala (Fabricius), Chrysomya (Ceylonomyia) nigripes Aubertin, Chrysomya pinguis (Walker), Chrysomya (Achoetandrus) rufifacies (Macquart), Hemipyrellia ligurriens (Wiedemann), Lucilia cuprina (Wiedemann), and Lucilia porphyrina (Walker). Based on the posterior spiracles geometry, the cross-validation revealed a relatively high percentage of correct classification in most species, ranking from 86% to 100%. The results of this study confirmed that the geometric morphometric (GM) analysis of posterior spiracles might be utilized as a larva identification tool. Therefore, this GM method represents one way of overcoming difficulties with the identification of blow fly larvae and can support further studies of these flies. [Display omitted]
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2024.107126