Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice

Key message Transgene with recombination sites to address biosafety concerns engineered into lettuce to produce EspB and γ -intimin C280 for oral vaccination against EHEC O157:H7. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, s...

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Bibliographic Details
Published in:Theoretical and applied genetics 2023-10, Vol.136 (10), p.214-214, Article 214
Main Authors: Su, Yun-Lin, Larzábal, Mariano, Song, Huan, Cheng, Tianfang, Wang, Yufang, Smith, Libia Yael, Cataldi, Angel Adrian, Ow, David W.
Format: Article
Language:English
Subjects:
Agriculture
Animals
Antigens
Biochemistry
Biomedical and Life Sciences
biosafety
Biotechnology
Cattle
E coli
edible vaccines
Enterohemorrhagic Escherichia coli
Escherichia coli
Escherichia coli infections
Escherichia coli O157
farms
food pathogens
Genetic aspects
genetically modified organisms
Immune response
Immunoglobulin A
Intimin
Lactuca
Leaves
Lettuce
Life Sciences
Mice
Mucosal immunity
oral vaccination
Oral vaccines
Original Article
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Leaves
Pollen
Prevention
Recombination
Risk factors
Testing
Transgenes
Transgenic mice
Vaccines
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Summary:Key message Transgene with recombination sites to address biosafety concerns engineered into lettuce to produce EspB and γ -intimin C280 for oral vaccination against EHEC O157:H7. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing 
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-023-04460-5