Construction and application of highly efficient waste cooking oil degrading bacteria consortium in oily wastewater

• Published in: Environmental science and pollution research international 2023-12, Vol.30 (60), p.125677-125688
• Main Authors: Zhao, Zhuo-qun, Yang, Jian, Chen, Heng-yuan, Wang, Wen-fan, Lian, Xiao-jian, Xie, Xiao-jie, Wang, Min, Yu, Ke-fei, Zheng, Hua-bao
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Bacteria - metabolism; Biodegradation; Biodegradation, Environmental; carboxylic ester hydrolases; chemical oxygen demand; Cooking; Cooking oils; Degradation; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; microbial communities; Microorganisms; Oil wastes; Oils; Phylogeny; Pseudomonas; Raoultella terrigena; Research Article; RNA, Ribosomal, 16S - metabolism; rRNA 16S; Sequence analysis; Strains (organisms); Waste Water Technology; Wastewater; Wastewater treatment; Water Management; Water Pollution Control
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-023-31107-1

The treatment of cooking oil wastewater is an urgent issue need to be solved. We aimed to screen for efficient oil-degrading bacteria and develop a new microbial agent for degrading waste cooking oil in oily wastewater. Three extremely effective oil-degrading bacteria, known as YZQ-1, YZQ-3, and YZQ-4, were found by the enrichment and acclimation of samples from various sources and separation using oil degradation plates. The 16S rRNA sequencing analysis and phylogenetic tree construction showed that the three strains were Bacillus tropicus , Pseudomonas multiresinivorans, and Raoultella terrigena . Under optimal degradation conditions, the maximal degradation rates were 67.30 ± 3.69%, 89.65 ± 1.08%, and 79.60 ± 5.30%, respectively, for YZQ-1, YZQ-3, and YZQ-4. Lipase activity was highest for YZQ-3, reaching 94.82 ± 12.89 U/L. The best bacterial alliance was obtained by adding equal numbers of microbial cells from the three strains. Moreover, when this bacterial alliance was applied to oily wastewater, the degradation rate of waste cooking oil was 61.13 ± 7.30% (3.67% ± 2.13% in the control group), and COD removal was 62.4% ± 5.65% (55.60% ± 0.71% in the control group) in 72 h. Microbial community analysis results showed YZQ-1 and YZQ-3 were adaptable to wastewater and could coexist with local bacteria, whereas YZQ-4 could not survive in wastewater. Therefore, the combination of YZQ-1 and YZQ-3 can efficiently degrade oil and shows great potential for oily wastewater treatment. Graphical Abstract