Rapid Simultaneous Determination of Cyanide, Azide, and Ethanol in Whole Blood Using Headspace Gas Chromatography-Mass Spectrometry

The rapid detection and quantification of toxic substances and alcohol in biological specimens stand as imperative tasks, particularly in instances of unnatural demise and poisoning incidents. In this study, we developed a high-throughput simultaneous method for analyzing cyanide, azide, and ethanol...

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Bibliographic Details
Published in:Chromatographia 2023-12, Vol.86 (11-12), p.701-706
Main Authors: Murakami, Takaya, Iwamuro, Yoshiaki, Sakamoto, Yuki, Minami, Eriko, Ishimaru, Reiko, Tsuchihashi, Hitoshi, Chinaka, Satoshi
Format: Article
Language:English
Subjects:
Analytical Chemistry
Ascorbic acid
azides
Blood
Chemistry
Chemistry and Materials Science
Chromatography
Correlation coefficients
cyanides
Ethanol
forensic sciences
Gas chromatography
gas chromatography-mass spectrometry
headspace analysis
Laboratory Medicine
Mass spectrometry
Pharmacy
Phosphoric acid
Proteomics
rapid methods
Scientific imaging
Short Communication
standard deviation
toxicity
Toxicology
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Summary:The rapid detection and quantification of toxic substances and alcohol in biological specimens stand as imperative tasks, particularly in instances of unnatural demise and poisoning incidents. In this study, we developed a high-throughput simultaneous method for analyzing cyanide, azide, and ethanol in whole blood. This methodology involves a simple manual pretreatment step, entailing the sequential addition of an internal standard, ascorbic acid, and phosphoric acid to whole blood samples (within 1 min), followed by headspace gas chromatography-mass spectrometry analysis (within 14 min). The method exhibits high linearity (correlation coefficients, > 0.9996), sensitivities of ng/mL-level detection, and satisfactory precision (relative standard deviation of less than 5.8% for cyanide, 8.8% for azide, and 2.9% for ethanol) and trueness (relative error within 7.3% for cyanide, 17% for azide, and 6.9% for ethanol). The proposed method, which does not require complex analytical techniques and possesses several advantages such as rapidity, simplicity, and high sensitivity and selectivity, is expected to contribute to the practical routine analysis in forensic and toxicological fields. Graphical Abstract
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-023-04289-6