Muscle Transcriptome Sequencing Revealed Thermal Stress–Responsive Regulatory Genes in Farmed Rohu, Labeo rohita (Hamilton, 1822)

Rohu, Labeo rohita , is one of the most important aquaculture species in the Indian subcontinent. Understanding the molecular-level physiological responses to thermal stress or climate change is essential. In the present work, transcriptome sequencing was carried out in the muscle tissue of the rohu...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2023-12, Vol.25 (6), p.1057-1075
Main Authors: Kumar, Pokanti Vinay, Rasal, Kiran D., Acharya, Arpit, Dey, Diganta, Sonwane, Arvind A., Reang, Dhalongsaih, Rajeshkannan, R., Pawar, Sachin S., Kurade, Nitin P., Bhendarkar, Mukesh P., Krishnani, Kishore K., Nagpure, Naresh S., Brahmane, Manoj P.
Format: Article
Language:English
Subjects:
acetyl coenzyme A
Angiopoietin
Aquaculture
Biological stress
Biomarkers
Biomedical and Life Sciences
c-Fos protein
Climate change
Crystallin
Engineering
Freshwater & Marine Ecology
Gene expression
gene expression regulation
Genes
Genomes
Heat stress
Heat tolerance
High temperature
Hsp70 protein
Immunity
India
Labeo rohita
Life Sciences
Microbiology
muscle tissues
Muscles
Nucleotide sequence
Physiological responses
Polyculture (aquaculture)
Proteins
proto-oncogenes
sequence analysis
Sequencing
temperature
Temperature effects
Thermal stress
Tissue
transcriptome
Transcriptomes
Zoology
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Summary:Rohu, Labeo rohita , is one of the most important aquaculture species in the Indian subcontinent. Understanding the molecular-level physiological responses to thermal stress or climate change is essential. In the present work, transcriptome sequencing was carried out in the muscle tissue of the rohu in response to heat stress (35 °C) in comparison with the control (28 °C). A total of 125 Gb of sequence data was generated, and the raw-reads were filtered and trimmed, which resulted in 484 million quality reads. Reference-based assembly of reads was performed using L. rohita genome, and a total of 90.17% of reads were successfully mapped. A total of 37,462 contigs were assembled with an N50 value of 1854. The differential expression analysis revealed a total of 107 differentially expressed genes (DEGs) (15 up-, 37 down-, and 55 neutrally regulated) as compared to the control group (Log2FC > 2, P  
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-023-10259-8