The Bactericidal and Antibiofilm Effects of New Liposomes Containing Vancomycin Formulation Against Clinical Biofilm Positive Staphylococcus aureus Isolates

The aim of present study was to prepare liposomes containing vancomycin (Lipo-VAN) formulation, and to evaluate the anti-biofilm effect of Lipo-VAN on the expression of icaA genes in clinical strains of Staphylococcus aureus . Lipo-VAN was synthesized using thin-layer hydration method. Formulation n...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2023-12, Vol.59 (6), p.824-832
Main Authors: Shiri, M., Ashrafi, F.
Format: Article
Language:English
Subjects:
Antibiotics
antimicrobial properties
Biochemistry
biofilm
Biofilms
Biomedical and Life Sciences
Gene expression
Life Sciences
Liposomes
Medical Microbiology
Microbiology
Polydispersity
Staphylococcus aureus
Vancomycin
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Summary:The aim of present study was to prepare liposomes containing vancomycin (Lipo-VAN) formulation, and to evaluate the anti-biofilm effect of Lipo-VAN on the expression of icaA genes in clinical strains of Staphylococcus aureus . Lipo-VAN was synthesized using thin-layer hydration method. Formulation no. 3 (F3) with the lowest size of 178.1 nm and polydispersity index value of 0.163 and with the highest efficiency for entrapping drug (65.49%) was selected as an optimal formulation. The formulations were tested for bactericidal and antibiofilm effects against S. aureus strains. The MIC and MBC obtained for the Lipo-VAN against clinical strains were 7.81–62.5 and 15.62–125 µg/mL, respectively. In addition, the reduction in biofilm formation in strains treated with Lipo-VAN was more significant rather than in the VAN-treated and control groups, which was demonstrated with down-regulation of icaA gene expression. Lipo-VAN formulation exhibited elevated antimicrobial and anti-biofilm activity against clinical S. aureus strains.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683823060157