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Serological and molecular detection of Toxoplasma gondii and Neospora caninum in free-ranging rats from Nagpur, India
Toxoplasma gondii and Neospora caninum are cyst-forming coccidian parasites that infect both wild and domestic non-felids as intermediate hosts, with rodents serving as important reservoir hosts during their life cycles. This study was aimed at investigating T . gondii and N . caninum infections and...
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| Published in: | Parasitology research (1987) 2024-01, Vol.123 (1), p.63-63, Article 63 |
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| container_end_page | 63 |
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| container_start_page | 63 |
| container_title | Parasitology research (1987) |
| container_volume | 123 |
| creator | Mishra, Veena Mitra, Pallabi Barbuddhe, Shruti Thorat, Yogesh Chavan, Kailas Shinde, Shilpshri Chaudhari, Sandeep Khan, Waqar Deshmukh, Abhijit S. |
| description | Toxoplasma gondii
and
Neospora caninum
are cyst-forming coccidian parasites that infect both wild and domestic non-felids as intermediate hosts, with rodents serving as important reservoir hosts during their life cycles. This study was aimed at investigating
T
.
gondii
and
N
.
caninum
infections and identifying factors favouring
T
.
gondii
infection in free-ranging rats from India. A total of 181 rodents were trap-captured, and blood and brain samples were subsequently collected for serological and molecular examination of
T
.
gondii
and
N
.
caninum
. Antibodies against
T
.
gondii
and
N
.
caninum
were detected by MAT/NAT and IFAT in 13.8% (25/181) and 1.65% (3/181) of rodents, respectively. All three
N
.
caninum
samples positive by NAT/IFAT were also positive for ELISA, while for
T
.
gondii
, 19 of 25 MAT/IFAT positive samples were also positive for ELISA. The antibody titers (MAT/NAT/IFAT) of rodents seropositive for
T
.
gondii
ranged from 25 to 400, while those of rats seropositive for
N
.
caninum
ranged from 25 to 100. Also, using PCR, DNA from
T
.
gondii
(B1 gene) and
N
.
caninum
(NC5 gene) was found in 2.76% (5/181) of brain samples and 0.55% (1/181) of brain samples. All PCR positive samples were also seropositive. No mixed infections were observed in the serological and molecular detections. A Chi-square analysis revealed that older rats and rats living in urban areas are significantly associated with
T
.
gondii
infection; however, rodent species, gender, location, habitat types, and seasonality were statistically nonsignificant. Overall, this study demonstrated that
T
.
gondii
was widely distributed while
N
.
caninum
was less prevalent among free-ranging rats in the studied area. |
| doi_str_mv | 10.1007/s00436-023-08095-9 |
| format | article |
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and
Neospora caninum
are cyst-forming coccidian parasites that infect both wild and domestic non-felids as intermediate hosts, with rodents serving as important reservoir hosts during their life cycles. This study was aimed at investigating
T
.
gondii
and
N
.
caninum
infections and identifying factors favouring
T
.
gondii
infection in free-ranging rats from India. A total of 181 rodents were trap-captured, and blood and brain samples were subsequently collected for serological and molecular examination of
T
.
gondii
and
N
.
caninum
. Antibodies against
T
.
gondii
and
N
.
caninum
were detected by MAT/NAT and IFAT in 13.8% (25/181) and 1.65% (3/181) of rodents, respectively. All three
N
.
caninum
samples positive by NAT/IFAT were also positive for ELISA, while for
T
.
gondii
, 19 of 25 MAT/IFAT positive samples were also positive for ELISA. The antibody titers (MAT/NAT/IFAT) of rodents seropositive for
T
.
gondii
ranged from 25 to 400, while those of rats seropositive for
N
.
caninum
ranged from 25 to 100. Also, using PCR, DNA from
T
.
gondii
(B1 gene) and
N
.
caninum
(NC5 gene) was found in 2.76% (5/181) of brain samples and 0.55% (1/181) of brain samples. All PCR positive samples were also seropositive. No mixed infections were observed in the serological and molecular detections. A Chi-square analysis revealed that older rats and rats living in urban areas are significantly associated with
T
.
gondii
infection; however, rodent species, gender, location, habitat types, and seasonality were statistically nonsignificant. Overall, this study demonstrated that
T
.
gondii
was widely distributed while
N
.
caninum
was less prevalent among free-ranging rats in the studied area.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-023-08095-9</identifier><identifier>PMID: 38114841</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Antibodies ; Antibodies, Protozoan ; B1 gene ; Biomedical and Life Sciences ; Biomedicine ; blood ; brain ; chi-square distribution ; Coccidiosis - epidemiology ; Coccidiosis - veterinary ; DNA ; Enzyme-linked immunosorbent assay ; gender ; genes ; habitats ; Health aspects ; Immunology ; India ; India - epidemiology ; Infection ; Life cycles ; Medical Microbiology ; Medical research ; Medicine, Experimental ; Microbiology ; Neospora ; Neospora caninum ; Polymerase chain reaction ; Protozoa ; Rats ; Rodentia ; Rodents ; Seroepidemiologic Studies ; Serology ; seroprevalence ; species ; Toxoplasma - genetics ; Toxoplasma gondii ; Toxoplasmosis, Animal - diagnosis ; Toxoplasmosis, Animal - epidemiology ; Viral antibodies</subject><ispartof>Parasitology research (1987), 2024-01, Vol.123 (1), p.63-63, Article 63</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2024 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-bd5f4e4788991f730af475748a727dc4613f121d7633d6035593fbfd537f7dbf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38114841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mishra, Veena</creatorcontrib><creatorcontrib>Mitra, Pallabi</creatorcontrib><creatorcontrib>Barbuddhe, Shruti</creatorcontrib><creatorcontrib>Thorat, Yogesh</creatorcontrib><creatorcontrib>Chavan, Kailas</creatorcontrib><creatorcontrib>Shinde, Shilpshri</creatorcontrib><creatorcontrib>Chaudhari, Sandeep</creatorcontrib><creatorcontrib>Khan, Waqar</creatorcontrib><creatorcontrib>Deshmukh, Abhijit S.</creatorcontrib><title>Serological and molecular detection of Toxoplasma gondii and Neospora caninum in free-ranging rats from Nagpur, India</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Toxoplasma gondii
and
Neospora caninum
are cyst-forming coccidian parasites that infect both wild and domestic non-felids as intermediate hosts, with rodents serving as important reservoir hosts during their life cycles. This study was aimed at investigating
T
.
gondii
and
N
.
caninum
infections and identifying factors favouring
T
.
gondii
infection in free-ranging rats from India. A total of 181 rodents were trap-captured, and blood and brain samples were subsequently collected for serological and molecular examination of
T
.
gondii
and
N
.
caninum
. Antibodies against
T
.
gondii
and
N
.
caninum
were detected by MAT/NAT and IFAT in 13.8% (25/181) and 1.65% (3/181) of rodents, respectively. All three
N
.
caninum
samples positive by NAT/IFAT were also positive for ELISA, while for
T
.
gondii
, 19 of 25 MAT/IFAT positive samples were also positive for ELISA. The antibody titers (MAT/NAT/IFAT) of rodents seropositive for
T
.
gondii
ranged from 25 to 400, while those of rats seropositive for
N
.
caninum
ranged from 25 to 100. Also, using PCR, DNA from
T
.
gondii
(B1 gene) and
N
.
caninum
(NC5 gene) was found in 2.76% (5/181) of brain samples and 0.55% (1/181) of brain samples. All PCR positive samples were also seropositive. No mixed infections were observed in the serological and molecular detections. A Chi-square analysis revealed that older rats and rats living in urban areas are significantly associated with
T
.
gondii
infection; however, rodent species, gender, location, habitat types, and seasonality were statistically nonsignificant. Overall, this study demonstrated that
T
.
gondii
was widely distributed while
N
.
caninum
was less prevalent among free-ranging rats in the studied area.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Protozoan</subject><subject>B1 gene</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>blood</subject><subject>brain</subject><subject>chi-square distribution</subject><subject>Coccidiosis - epidemiology</subject><subject>Coccidiosis - veterinary</subject><subject>DNA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>gender</subject><subject>genes</subject><subject>habitats</subject><subject>Health aspects</subject><subject>Immunology</subject><subject>India</subject><subject>India - epidemiology</subject><subject>Infection</subject><subject>Life cycles</subject><subject>Medical Microbiology</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microbiology</subject><subject>Neospora</subject><subject>Neospora caninum</subject><subject>Polymerase chain reaction</subject><subject>Protozoa</subject><subject>Rats</subject><subject>Rodentia</subject><subject>Rodents</subject><subject>Seroepidemiologic Studies</subject><subject>Serology</subject><subject>seroprevalence</subject><subject>species</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - diagnosis</subject><subject>Toxoplasmosis, Animal - epidemiology</subject><subject>Viral antibodies</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkk1rFTEYhYNY7LX6B1xIwI0LpyaTr8myFFsLpS6s65CbjyElk4zJDNR_b25vW1GkJYuEN885OYEDwDuMjjFC4nNFiBLeoZ50aECSdfIF2GBK-g5Lxl6CDZLtjDAmh-B1rTcIYcEpfQUOyYAxHSjegPW7KznmMRgdoU4WTjk6s0ZdoHWLM0vICWYPr_NtnqOuk4ZjTjaEO_jK5TrnoqHRKaR1giFBX5zrik5jSCMseqltkid4pcd5LZ_gRRPrN-DA61jd2_v9CPw4-3J9-rW7_HZ-cXpy2Rna86XbWuapo2IYpMReEKQ9FUzQQYteWEM5Jh732ApOiOWIMCaJ33rLiPDCbj05Ah_3vnPJP1dXFzWFalyMOrm8VkUwI82PI_4s2ktEcXuA7dAP_6A3eS2pfWRHkRaWtkCP1KijUyH5vBRtdqbqRAg-tJC8b9Txf6i2rJuCycn50OZ_Cfq9wJRca3FezSVMuvxSGKldLdS-FqrVQt3VQskmen-feN1Ozj5KHnrQALIHartKoyt_vvSE7W8US8BX</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Mishra, Veena</creator><creator>Mitra, Pallabi</creator><creator>Barbuddhe, Shruti</creator><creator>Thorat, Yogesh</creator><creator>Chavan, Kailas</creator><creator>Shinde, Shilpshri</creator><creator>Chaudhari, Sandeep</creator><creator>Khan, Waqar</creator><creator>Deshmukh, Abhijit S.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240101</creationdate><title>Serological and molecular detection of Toxoplasma gondii and Neospora caninum in free-ranging rats from Nagpur, India</title><author>Mishra, Veena ; Mitra, Pallabi ; Barbuddhe, Shruti ; Thorat, Yogesh ; Chavan, Kailas ; Shinde, Shilpshri ; Chaudhari, Sandeep ; Khan, Waqar ; Deshmukh, Abhijit S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-bd5f4e4788991f730af475748a727dc4613f121d7633d6035593fbfd537f7dbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Protozoan</topic><topic>B1 gene</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>blood</topic><topic>brain</topic><topic>chi-square distribution</topic><topic>Coccidiosis - epidemiology</topic><topic>Coccidiosis - veterinary</topic><topic>DNA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>gender</topic><topic>genes</topic><topic>habitats</topic><topic>Health aspects</topic><topic>Immunology</topic><topic>India</topic><topic>India - epidemiology</topic><topic>Infection</topic><topic>Life cycles</topic><topic>Medical Microbiology</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Microbiology</topic><topic>Neospora</topic><topic>Neospora caninum</topic><topic>Polymerase chain reaction</topic><topic>Protozoa</topic><topic>Rats</topic><topic>Rodentia</topic><topic>Rodents</topic><topic>Seroepidemiologic Studies</topic><topic>Serology</topic><topic>seroprevalence</topic><topic>species</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - diagnosis</topic><topic>Toxoplasmosis, Animal - epidemiology</topic><topic>Viral antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mishra, Veena</creatorcontrib><creatorcontrib>Mitra, Pallabi</creatorcontrib><creatorcontrib>Barbuddhe, Shruti</creatorcontrib><creatorcontrib>Thorat, Yogesh</creatorcontrib><creatorcontrib>Chavan, Kailas</creatorcontrib><creatorcontrib>Shinde, Shilpshri</creatorcontrib><creatorcontrib>Chaudhari, Sandeep</creatorcontrib><creatorcontrib>Khan, Waqar</creatorcontrib><creatorcontrib>Deshmukh, Abhijit S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mishra, Veena</au><au>Mitra, Pallabi</au><au>Barbuddhe, Shruti</au><au>Thorat, Yogesh</au><au>Chavan, Kailas</au><au>Shinde, Shilpshri</au><au>Chaudhari, Sandeep</au><au>Khan, Waqar</au><au>Deshmukh, Abhijit S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serological and molecular detection of Toxoplasma gondii and Neospora caninum in free-ranging rats from Nagpur, India</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>123</volume><issue>1</issue><spage>63</spage><epage>63</epage><pages>63-63</pages><artnum>63</artnum><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Toxoplasma gondii
and
Neospora caninum
are cyst-forming coccidian parasites that infect both wild and domestic non-felids as intermediate hosts, with rodents serving as important reservoir hosts during their life cycles. This study was aimed at investigating
T
.
gondii
and
N
.
caninum
infections and identifying factors favouring
T
.
gondii
infection in free-ranging rats from India. A total of 181 rodents were trap-captured, and blood and brain samples were subsequently collected for serological and molecular examination of
T
.
gondii
and
N
.
caninum
. Antibodies against
T
.
gondii
and
N
.
caninum
were detected by MAT/NAT and IFAT in 13.8% (25/181) and 1.65% (3/181) of rodents, respectively. All three
N
.
caninum
samples positive by NAT/IFAT were also positive for ELISA, while for
T
.
gondii
, 19 of 25 MAT/IFAT positive samples were also positive for ELISA. The antibody titers (MAT/NAT/IFAT) of rodents seropositive for
T
.
gondii
ranged from 25 to 400, while those of rats seropositive for
N
.
caninum
ranged from 25 to 100. Also, using PCR, DNA from
T
.
gondii
(B1 gene) and
N
.
caninum
(NC5 gene) was found in 2.76% (5/181) of brain samples and 0.55% (1/181) of brain samples. All PCR positive samples were also seropositive. No mixed infections were observed in the serological and molecular detections. A Chi-square analysis revealed that older rats and rats living in urban areas are significantly associated with
T
.
gondii
infection; however, rodent species, gender, location, habitat types, and seasonality were statistically nonsignificant. Overall, this study demonstrated that
T
.
gondii
was widely distributed while
N
.
caninum
was less prevalent among free-ranging rats in the studied area.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38114841</pmid><doi>10.1007/s00436-023-08095-9</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2024-01, Vol.123 (1), p.63-63, Article 63 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153574606 |
| source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
| subjects | Animals Antibodies Antibodies, Protozoan B1 gene Biomedical and Life Sciences Biomedicine blood brain chi-square distribution Coccidiosis - epidemiology Coccidiosis - veterinary DNA Enzyme-linked immunosorbent assay gender genes habitats Health aspects Immunology India India - epidemiology Infection Life cycles Medical Microbiology Medical research Medicine, Experimental Microbiology Neospora Neospora caninum Polymerase chain reaction Protozoa Rats Rodentia Rodents Seroepidemiologic Studies Serology seroprevalence species Toxoplasma - genetics Toxoplasma gondii Toxoplasmosis, Animal - diagnosis Toxoplasmosis, Animal - epidemiology Viral antibodies |
| title | Serological and molecular detection of Toxoplasma gondii and Neospora caninum in free-ranging rats from Nagpur, India |
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