The grapevine miR827a regulates the synthesis of stilbenes by targeting VqMYB14 and gives rise to susceptibility in plant immunity

• Published in: Theoretical and applied genetics 2024-04, Vol.137 (4), p.95-95, Article 95
• Main Authors: Luo, Yangyang, Wang, Linxia, Zhu, Jie, Tian, Jingwen, You, Lin, Luo, Qin, Li, Jia, Yao, Qian, Duan, Dong
• Format: Article
• Language: English
• Subjects: Agriculture; Arabidopsis - genetics; Arabidopsis thaliana; Biochemistry; Biomedical and Life Sciences; Biotechnology; China; Crops; Disease Resistance - genetics; Economic importance; elicitors; ethylene; Flagellin; Fruit cultivation; fruits; Gene Expression Regulation, Plant; genes; genetically modified organisms; grapes; immunity; Life Sciences; luciferase; microRNA; MicroRNAs; miRNA; Molecular modelling; Original Article; pathogens; peptides; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Genetics and Genomics; Plant immunity; Plant Immunity - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Protoplasts; Pseudomonas syringae pv. tomato; Stilbene; Stilbenes; stress response; stress tolerance; transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; transcriptome; Transcriptomes; Vitis - genetics; Vitis heyneana; Vitis quinquangularis; Vitis vinifera; yeasts
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-024-04599-9

Grapevine ( Vitis vinifera L.) is an economically important fruit crop cultivated worldwide. In China, grapevine cultivation is very extensive, and a few Vitis grapes have excellent pathogen and stress resistance, but the molecular mechanisms underlying the grapevine response to stress remain unclear. In this study, a microRNA (miRNA; miR827a), which negatively regulates its target gene VqMYB14 , a key regulatory role in the synthesis of stilbenes, was identified in Vitis quinquangularis ( V. quinquangularis ) using transcriptome sequencing. Using overexpression and silencing approaches, we found that miR827a regulates the synthesis of stilbenes by targeting VqMYB14 . We used flagellin N-terminal 22-amino-acid peptide (flg22), the representative elicitor in plant basal immunity, as the elicitor to verify whether miR827a is involved in the basal immunity of V. quinquangularis . Furthermore, the promoter activity of miR827a was alleviated in transgenic grape protoplasts and Arabidopsis thaliana following treatment with flg22 and Pseudomonas syringae pv. Tomato DC3000 ( Pst DC3000), respectively. In addition, yeast one-hybrid and dual luciferase reporter assay revealed that the ethylene transcription factor VqERF057 acted as a key regulator in the inhibition of miR827a transcription. These results will contribute to the understanding of the biological functions of miR827a in grapevine and clarify the molecular mechanism of the interaction between miR827a and VqMYB14 .