Electrochemical sensor based on ionic liquid for detection of lactose content in dairy products

This paper presents the development of an electrochemical sensor based on a modified ionic liquid, 3-Amine-N-[3-(N-pyrrole)propyl]imidazole bromide (APPIBr), for the detection of lactose content in dairy products. The sensor utilizes cellobiose dehydrogenase (CDH) as the catalytic enzyme for lactose...

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Bibliographic Details
Published in:Journal of food measurement & characterization 2024, Vol.18 (1), p.313-319
Main Author: Wu, Hui
Format: Article
Language:English
Subjects:
Cellobiose
Cellobiose dehydrogenase
Chemical sensors
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Dairy products
Dehydrogenases
detection limit
Electrochemistry
Electrodes
Electrons
Engineering
Enzymes
Food Science
Imidazole
Ionic liquids
Lactose
Lactose intolerance
Original Paper
Oxidation
Sample preparation
Sensors
sensors (equipment)
Solvents
Spectrum analysis
Vibration
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Summary:This paper presents the development of an electrochemical sensor based on a modified ionic liquid, 3-Amine-N-[3-(N-pyrrole)propyl]imidazole bromide (APPIBr), for the detection of lactose content in dairy products. The sensor utilizes cellobiose dehydrogenase (CDH) as the catalytic enzyme for lactose oxidation. The APPIBr/CDH-modified electrode exhibits excellent electrocatalytic activity, enabling sensitive and selective lactose detection. The sensor offers a wide linear range of 50 µM to 3 mM and a low detection limit of 0.58 µM. The developed sensor eliminates the need for complex sample preparation steps, providing rapid and on-site analysis of lactose in dairy products. Compared to traditional lactose detection methods such as HPLC, the sensor demonstrates comparable accuracy and repeatability. The findings highlight the potential of the APPIBr/CDH-based electrochemical sensor for practical applications in the analysis of lactose content in dairy products.
ISSN:2193-4126
2193-4134
DOI:10.1007/s11694-023-02181-3