MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression

• Published in: Functional & integrative genomics 2024-04, Vol.24 (2), p.54-54, Article 54
• Main Authors: Zhu, Kai, Zhang, Yan, Li, DongDong, Xie, MingZhong, Jiang, HuaCai, Zhang, KaiQuan, Lei, Yang, Chen, GuangYou
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Apoptosis; Apoptosis - genetics; Arthritis; Biochemistry; Bioinformatics; Biomarkers; Biomedical and Life Sciences; Bone Neoplasms; Cartilage; Cartilage diseases; Cell Biology; Cell growth; Cell proliferation; Cell Proliferation - genetics; Cholecystokinin; Chondrocytes; Chondrosarcoma; Chondrosarcoma - genetics; Clinical outcomes; Clinical trials; Flow cytometry; Gene expression; Gene therapy; genomics; Humans; Immunohistochemistry; Intervention; Life Sciences; Microbial Genetics and Genomics; microRNA; MicroRNAs - genetics; MicroRNAs - metabolism; Original Article; Osteoarthritis; Osteoarthritis - genetics; Plant Genetics and Genomics; PTEN protein; Reporter gene; reporter genes; Targeted cancer therapy; Tensins; Therapeutic targets; Western blotting
• ISSN: 1438-793X; 1438-7948
• DOI: 10.1007/s10142-024-01327-w

Despite substantial progress in clinical trials of osteoarthritis (OA) gene therapy, the prevalence of OA is still on the rise. MiRNAs have a potential biomarker and therapeutic target for OA. OA cartilage and chondrosarcoma cells were studied to determine the role of miR-29a-3p and PTEN. OA cartilage and human chondrosarcoma cells (SW1353) were obtained. miR-29a-3p and PTEN signature expression was determined by RT-qPCR. The binding relationship between miR-29a-3p and PTEN was investigated by dual-luciferase reporter gene and western blot assay. TUNEL, immunohistochemistry, CCK-8, and flow cytometry were utilized to determine the proliferation and apoptosis of SW1353 cells. This study indicated downregulation of miR-29a-3p expression and upregulation of PTEN expression in human OA primary chondrocytes or OA tissue samples, compared with the normal cartilage cells or tissues. PTEN expression was negatively correlated with miR-29a-3p expression, and miR-29a-3p targeted PTEN mechanistically. miR-29a-3p reduced SW1353 cell activity and proliferation and promoted cell apoptosis. However, the aforementioned effects could be reversed by downregulating PTEN. miR-29a-3p can stimulate chondrocyte proliferation and inhibit apoptosis by inhibiting PTEN expression.