Gordonibacter faecis sp. nov., producing urolithin C from ellagic acid, isolated from feces of healthy Korean subjects

A Gram-stain-positive, anaerobic, motile, and short rod-shaped bacterium, designated KGMB12511 T , was isolated from the feces of healthy Koreansubjects. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain KGMB12511 T was closely related to Gordonibacter pamelaeae 7-10-1-b T...

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Bibliographic Details
Published in:Archives of microbiology 2024-03, Vol.206 (3), p.108-108, Article 108
Main Authors: Kim, Han Sol, Kim, Ji-Sun, Suh, Min Kuk, Eom, Mi Kyung, Do, Hyo Eun, Lee, Ju Huck, Park, Seung-Hwan, Kang, Se Won, Lee, Dong Ho, Yoon, Hyuk, Lee, Je Hee, Lee, Jung-Sook
Format: Article
Language:English
Subjects:
Archives & records
bacteria
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
chemotaxonomy
Dimethyl acetals
DNA
Ecology
Ellagic Acid
Fatty acids
Feces
Gene sequencing
Genes
genome
Genomes
genus
Glycolipids
Humans
Hydrolyzable Tannins
Life Sciences
Lipids
Menaquinones
Microbial Ecology
Microbiology
New species
Nucleotide sequence
nucleotide sequences
Original Paper
phenotype
Phospholipids
Phylogenetics
Phylogeny
Polyphenols
Quinones
Republic of Korea
RNA, Ribosomal, 16S - genetics
rRNA 16S
species
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Summary:A Gram-stain-positive, anaerobic, motile, and short rod-shaped bacterium, designated KGMB12511 T , was isolated from the feces of healthy Koreansubjects. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain KGMB12511 T was closely related to Gordonibacter pamelaeae 7-10-1-b T (95.2%). The draft genome of KGMB12511 T comprised 33 contigs and 2,744 protein-coding genes. The DNA G + C content was 59.9% based on whole-genome sequences. The major cellular fatty acids (>10%) of strain KGMB12511 T were C 18:1 cis 9, C 18:1 cis 9 DMA (dimethylacetal), and C 16:0 DMA. The predominant polar lipids included a diphosphatydilglycerol, four glycolipids, and an unidentified phospholipid. The major respiratory quinones were menaquinone 6 (MK-6) and monomethylmenaquinone 6 (MMK-6). Furthermore, HPLC analysis demonstrated the ability of strain KGMB12511 T to convert ellagic acid into urolithin. Based on a comprehensive analysis of phenotypic, chemotaxonomic, and phylogenetic data, strain KGMB12511 T represents a novel species in the genus Gordonibacter . The type strain is KGMB12511 T (= KCTC 25343 T  = NBRC 116190 T ).
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-024-03844-5