Learning the native pond odor as one of the mechanisms of olfactory orientation in juvenile smooth newt Lissotriton vulgaris

• Published in: Journal of Comparative Physiology 2024, Vol.210 (1), p.57-63
• Main Authors: Vyatkin, Yaroslav Aleksandrovich, Shakhparonov, Vladimir Vladimirovich
• Format: Article
• Language: English
• Subjects: Amphibians; Animal Physiology; Animals; Anura; Biomedical and Life Sciences; Breeding sites; Developmental stages; Juveniles; Larvae; Larval development; Learning; Life Sciences; Lissotriton vulgaris; memory; Metamorphosis; Morpholine; Morpholines; Neurosciences; Odor recognition; Odorants; Odors; Olfaction; Orientation; Original Paper; Ponds; salamanders and newts; Salamandridae - physiology; Smell; surface water; Water; Water bodies; Zoology
• ISSN: 0340-7594; 1432-1351
• DOI: 10.1007/s00359-023-01640-y

Olfaction is an important mechanism of orientation in amphibians toward the breeding site. It is known that anurans can memorize the odor of the native pond during larval development and prefer this odor prior to the beginning of dispersion. However, such a mechanism in urodeles has not been studied yet. We conducted experiments on recognition of the odor of a native water body in juveniles of the smooth newt Lissotriton vulgaris . One group of larvae were reared in pure water (control), the other group in water with morpholine (10 –7  mol/L). A few days after metamorphosis, the newts were tested under paired-choice conditions in a T-maze. A total of 73 newts from the experimental group and 47 newts from the control group were tested. The results of the experiment show that the newts in the experimental group preferred the morpholine solution, whereas the individuals of the control group made the choice randomly. We conclude that newts can learn the odor of the environment in which they developed and use this memory for orientation in later stages of life.