Ferruginous components of particulate matters in subway environments, α-Fe2O3 or Fe3O4, exacerbates allergies

• Published in: Environmental pollution (1987) 2024-08, Vol.355, p.124195-124195, Article 124195
• Main Authors: Watanabe, Hikari, Honda, Akiko, Ichinose, Takamichi, Ishikawa, Raga, Miyasaka, Natsuko, Nagao, Megumi, Wang, Zaoshi, Owokoniran, Oluwatoyin Hannah, Qiu, Binyang, Higaki, Yuya, Liu, Wei, Okuda, Tomoaki, Matsuda, Tomonari, Takano, Hirohisa
• Format: Article
• Language: English
• Subjects: Allergy; antigen presentation; chemokines; death; epithelium; hypersensitivity; immune response; inflammation; Inflammatory response; interleukin-13; Iron oxide; iron oxides; lactate dehydrogenase; ligands; lungs; mucus; ovalbumin; Particulate matter; particulates; phagocytosis; pollution; Raman spectroscopy; Subway
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2024.124195

The respiratory effects of particulate matter (PM) in subway station platforms or tunnels have attracted considerable research attention. However, no studies have characterized the effects of subway PM on allergic immune responses. In this study, iron oxide (α-Fe2O3 and Fe3O4) particles—the main components of subway PM—were intratracheally administered to BALB/c mice where ovalbumin (OVA) induced allergic pulmonary inflammation. Iron oxide particles enhanced OVA-induced eosinophil recruitment around the bronchi and mucus production from airway epithelium. The concentrations of type 2 cytokines, namely, interleukin (IL)-5 and IL-13, in bronchial alveolar lavage fluids were increased by iron oxide particles. Iron oxide particles also increased the number of type 2 innate lymphoid cells and CD86+ cells in the lung. Moreover, phagocytosis of particles in lung cells was confirmed by Raman spectroscopy. In a subsequent in vitro study, bone marrow-derived antigen-presenting cells (APCs) isolated from NC/Nga mice were exposed to iron oxide particles and OVA. They were also exposed to outdoor ambient PM: Vehicle Exhaust Particulates (VEP) and Urban Aerosols (UA) as references. Iron oxide particles promoted the release of lactate dehydrogenase, C-X-C motif chemokine ligand 1 and IL-1α from APCs, which tended to be stronger than those of VEP. These results suggest that iron oxide particles enhance antigen presentation in the lungs, promoting allergic immune response in mice; iron oxide particles-induced death and inflammatory response of APCs can contribute to allergy exacerbation. Although iron oxide particles do not contain various compounds like VEP, iron oxide alone may have sufficient influence. [Display omitted] •α-Fe2O3/Fe3O4 particles were used as the components of subway particulate matters.•Iron oxide particles exacerbated allergic airway inflammation in mice.•Iron oxide particle-enhanced antigen-presenting can trigger allergies.•Iron oxide particles increased type 2 innate lymphoid cells in murine lungs.•α-Fe2O3 and Fe3O4 were detected in lung macrophages by Raman spectroscopy.