Molecular Detection of Hemoplasma in animals in Tamil Nadu, India and Hemoplasma genome analysis

• Published in: Veterinary research communications 2024-04, Vol.48 (2), p.955-968
• Main Authors: Murugesan, Ananda Chitra, Kumaragurubaran, Karthik, Gunasekaran, Kavin, Murugasamy, Sabari Arul, Arunachalam, Shanmugasundaram, Annamalai, Raman, Ragothaman, Venkataramanan, Ramaswamy, Sridhar
• Format: Article
• Language: English
• Subjects: adhesion; Anemia; antibiotic resistance; Antimicrobial resistance; Biomedical and Life Sciences; buffaloes; Candidatus Mycoplasma haemobos; Cattle; CRISPR; CRISPR-Cas systems; DNA; Erythrocytes; Genes; Genomes; goats; Gram-positive bacteria; Hemolytic anemia; India; Life Sciences; Mycoplasma ovis; Mycoplasma wenyonii; Phosphopyruvate hydratase; rRNA 16S; sequence analysis; Sheep; Veterinary Medicine/Veterinary Science; Zoology
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1007/s11259-023-10263-2

Hemoplasma are small pleomorphic wall-less Gram-positive bacteria that infect erythrocytes of various mammalian hosts. They generally cause asymptomatic or chronic anaemia but occasionally causes overt life-threatening hemolytic anaemia. In the present study, 316 cattle, 115 sheep, 61 goats and 6 buffalo blood samples were collected from various villages or organized farms located in nine districts of Tamil Nadu to detect the hemoplasma by PCR. Overall prevalence of 43.04%, 65.22%, and 44.26% hemoplasma DNA was observed in cattle, sheep and goats, respectively. In total, 21 hemoplasma positive samples were sequenced for 16S rRNA gene which revealed 8 Mycoplasma wenyonii , 11 ‘Candidatus Mycoplasma haemobos ’ and one Mycoplasma ovis infection. Sheep blood samples from Chennai district were infected with ‘Ca. M. haemobos’ whereas sheep sample from Thiruvannamalai district was infected with M. wenyonii . At least 50% genes in the hemoplasma genomes were paralogous genes whose functions were not known. Only ‘Ca. M. haemolamae’ genome contained one primitive CRISPR system without any cas genes. Antimicrobial resistance genes (ARG) could not be identified in any of the hemoplasma genomes but homologous ARG were identified in all the genomes. Adhesion related gene EF-Tu was detected in all 14 hemoplasma genomes but enolase gene was detected only in ‘Ca. M. haemohominis’ SWG34-3 genome. This is the first report on the prevalence of hemoplasma infection in cattle, sheep and goat in India.