Multifunctional pH-responsive hydrogel dressings based on carboxymethyl chitosan: Synthesis, characterization fostering the wound healing

• Published in: Carbohydrate polymers 2024-10, Vol.341, p.122348, Article 122348
• Main Authors: Xiong, Mingxin, Chen, Yu, Hu, Han-Jian, Cheng, Hao, Li, Wei-Xiong, Tang, Shipeng, Hu, Xiaolong, Lan, Ling-Min, Zhang, Hongyan, Jiang, Gang-Biao
• Format: Article
• Language: English
• Subjects: adhesion; Animals; Anti-bacterial; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antibiotics; antioxidant activity; bacterial infections; Bandages; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Carboxymethyl chitosan; chitosan; Chitosan - analogs & derivatives; Chitosan - chemical synthesis; Chitosan - chemistry; Chitosan - pharmacology; coagulants; collagen; cross-linking reagents; drug resistance; granulation tissue; health services; Hydrogel dressing; hydrogels; Hydrogels - chemical synthesis; Hydrogels - chemistry; Hydrogels - pharmacology; Hydrogen-Ion Concentration; macrophages; Male; Mice; pH-responsive; plant extracts; polyvinylpyrrolidone; Povidone - chemistry; Self-adaptive; Skin - drug effects; Skin - pathology; Staphylococcus aureus - drug effects; Tannic acid; Wound Healing - drug effects
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2024.122348

Antibiotic abuse is increasing the present rate of drug-resistant bacterial wound infections, producing a significant healthcare burden globally. Herein, we prepared a pH-responsive CMCS/PVP/TA (CPT) multifunctional hydrogel dressing by embedding the natural plant extract TA as a nonantibiotic and cross-linking agent in carboxymethyl chitosan (CMCS) and polyvinylpyrrolidone (PVP) to prompt wound healing. The CPT hydrogel demonstrated excellent self-healing, self-adaptive, and adhesion properties to match different wound requirements. Importantly, this hydrogel showed pH sensitivity and exhibited good activity against resistant bacteria and antioxidant activity by releasing TA in case of bacterial infection (alkaline). Furthermore, the CPT hydrogel exhibited coagulant ability and could rapidly stop bleeding within 30 s. The biocompatible hydrogel effectively accelerated wound healing in a full-thickness skin defect model by thickening granulation tissue, increasing collagen deposition, vascular proliferation, and M2-type macrophage polarization. In conclusion, this study demonstrates that multifunctional CPT hydrogel offers a candidate material with potential applications for infected skin wound healing. [Display omitted]