Unraveling the effectiveness of Sargassum wightii against white spot syndrome virus in Paratelphusa hydrodomous: an in-vivo and in-silico approach

White spot syndrome virus (WSSV) is causing significant economic losses in shrimp farming. Envelope proteins, which interact with host cells during viral attachment, are potential targets for drug development. In this study, the antiviral activity of Sargassum wightii was assessed using in-vitro and...

Full description

Saved in:
Bibliographic Details
Published in:Fisheries science 2024-05, Vol.90 (3), p.397-408
Main Authors: Raja, Bharath, Radhakrishnan, Vidya
Format: Article
Language:English
Subjects:
Algae
Animals
Antiviral activity
Antiviral agents
antiviral properties
Aquaculture
Bio-assays
Biomedical and Life Sciences
computer simulation
Crustaceans
Disease
Drug development
Economic impact
Fish & Wildlife Biology & Management
Fisheries
Food Science
Freshwater & Marine Ecology
Histopathology
Infections
Life Sciences
Marine crustaceans
Medical research
Mortality
Original Article
Pathogens
Proteins
Sargassum wightii
Shellfish farming
shrimp
Shrimp culture
Vaccines
Viral envelope proteins
Viral infections
Virus attachment
White spot syndrome
White spot syndrome virus
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:White spot syndrome virus (WSSV) is causing significant economic losses in shrimp farming. Envelope proteins, which interact with host cells during viral attachment, are potential targets for drug development. In this study, the antiviral activity of Sargassum wightii was assessed using in-vitro and in-silico analyses. Crabs were injected with S. wightii extract and WSSV and were observed for 30 days post-infection. The antiviral activity was confirmed by bio-assay, histopathology, and in-silico analysis. GC–MS analysis identified 15 compounds in S. wightii , and in-silico docking revealed a strong interaction between VP28 and VP26 envelope proteins and stigmasta-4,22-dien-3-one. Molecular simulations validated the stability of the protein–ligand binding. These findings confirm the potential of S. wightii as a treatment for WSSV.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-024-01758-1