Undibacterium cyanobacteriorum sp. nov., an auxin-producing bacterium isolated from fresh water during cyanobacterial bloom period

• Published in: Antonie van Leeuwenhoek 2024-12, Vol.117 (1), p.99-99, Article 99
• Main Authors: Van Le, Ve, Ko, So-Ra, Nguyen, Loan Thi Thanh, Kim, Jin-Cheol, Shin, Yuna, Kim, Kyunghyun, Ahn, Chi-Yong
• Format: Article
• Language: English
• Subjects: Auxins; bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; chemotaxonomy; Cyanobacteria - classification; Cyanobacteria - genetics; Cyanobacteria - isolation & purification; Cyanobacteria - metabolism; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Fresh water; Fresh Water - microbiology; freshwater; Gene sequencing; genes; genus; Hybridization; Indoleacetic Acids - metabolism; Life Sciences; Medical Microbiology; Microbiology; New species; Nucleic Acid Hybridization; Nucleotide sequence; Nucleotides; Original Paper; phenotype; Phylogeny; Plant Sciences; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; sequence homology; Soil Science & Conservation; species; Water Microbiology
• ISSN: 0003-6072; 1572-9699; 1572-9699
• DOI: 10.1007/s10482-024-01992-8

A novel Gram-negative, white-pigmented, and auxin-producing strain, 20NA77.5 T , was isolated from fresh water during cyanobacterial bloom period. Pairwise comparison of the 16S rRNA gene sequences showed that strain 20NA77.5 T belonged to the genus Undibacterium and exhibited the highest sequence similarity to the type strains of Undibacterium danionis (98.00%), Undibacterium baiyunense (97.93%), Undibacterium macrobrachii (97.92%), and Undibacterium fentianense (97.71%) . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain 20NA77.5 T and its related type strains were below 79.93 and 23.80%, respectively. The predominant fatty acids (> 10% of the total fatty acids) were C 16:0 and summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ). The genomic DNA G + C content of strain 20NA77.5 T was found to be 48.61%. Based on the phylogenetic distinctness, chemotaxonomic features, and phenotypic features, strain 20NA77.5 T is considered to represent a novel species of the genus Undibacterium, for which the name Undibacterium cyanobacteriorum sp. nov is proposed. The type strain is 20NA77.5 T (= KCTC 8005 T  = LMG 33136 T ).