De novo synthesis of monounsaturated fatty acids modulates exosome-mediated lipid export from human granulosa cells

• Published in: Molecular and cellular endocrinology 2024-10, Vol.592, p.112317, Article 112317
• Main Authors: Fayezi, Shabnam, Oehms, Sophie, Wolff von Gudenberg, Helena, Ponnaiah, Maharajah, Lhomme, Marie, Strowitzki, Thomas, Germeyer, Ariane
• Format: Article
• Language: English
• Subjects: cell communication; cell cycle; cyclins; endocrinology; exosomes; exports; Fatty acids; fatty-acid synthase; humans; lipid content; Lipidomics; lipogenesis; liquid chromatography; mass spectrometry; nanoparticles; Oleate; oleic acid; oocytes; Ovarian follicle; quantitative polymerase chain reaction; stearoyl-CoA desaturase; transmission electron microscopy
• ISSN: 0303-7207; 1872-8057; 1872-8057
• DOI: 10.1016/j.mce.2024.112317

Ovarian somatic cells support the maturation and fertility of oocytes. Metabolic desaturation of fatty acids in these cells has a positive paracrine impact on the maturation of oocytes. We hypothesized that the enzyme stearoyl-CoA desaturase 1 (SCD1) in granulosa cells regulates the lipid cargo of exosomes secreted from these cells by maintaining the balance between saturated and unsaturated lipids. We investigated the effect of SCD1 on exosome lipid content in a cumulus-granulosa cell model under physiologically relevant in vitro conditions. Non-luteinized human COV434 granulosa cells were subjected to treatment with an inhibitor of SCD1 (SCDinhib) alone, in combination with oleic acid, or under control conditions. Subsequently, the exosomes were isolated and characterized via nanoparticle tracking analysis, transmission electron microscopy, and Western blotting. We used liquid chromatography mass spectrometry to investigate the lipidomic profiles. We used quantitative PCR with TaqMan primers to assess the expression of genes involved in lipogenesis and control of cell cycle progression. A trend toward exosome production was observed with a shift toward smaller exosome sizes in cells treated with SCD1inhib. This trend reached statistical significance when SCDinhib was combined with oleic acid supplementation. SCD1 inhibition led to the accumulation of saturated omega-6 lipids in exosomes. The latter effect was reversed by oleic acid supplementation, which also improved exosome production and suppressed the expression of fatty acid synthase and Cyclin D2. These findings underscore the critical role of de novo fatty acid desaturation in the regulation of the export of specific lipids through exosomes, with potential implications for controlling intercellular communication within the ovary. [Display omitted] •SCD inhibition increased the export of saturated lipids via exosomes.•Inhibition of SCD also led to an increase in the omega-6 lipid content of exosomes.•Oleic acid supplementation reversed the accumulation of omega-6 lipids in exosomes induced by inhibition of SCD1.