Megasporogenesis and megagametogenesis in Hydrocleys nymphoides, Alisma plantago-aquatica, and Sagittaria montevidensis (Alismataceae)

• Published in: Protoplasma 2024-07, Vol.261 (4), p.725-733
• Main Authors: Nicolau, Magali, Reposi, Sofía, Gotelli, Marina, Zarlavsky, Gabriela, Galati, Beatriz
• Format: Article
• Language: English
• Subjects: Alisma - chemistry; Alisma - cytology; Alisma plantago-aquatica; Alismataceae; Alismataceae - cytology; aquatic organisms; Biomedical and Life Sciences; Cell Biology; Developmental stages; family; Females; gametophytes; genus; histology; Hydrocleys; Hydrocleys nymphoides; Life Sciences; megasporogenesis; Nuclei; Original Article; Ovule - cytology; ovules; Phylogeny; Plant Sciences; Polygonum; Sagittaria - cytology; Sagittaria montevidensis; Species; Zoology
• ISSN: 0033-183X; 1615-6102; 1615-6102
• DOI: 10.1007/s00709-024-01930-5

Ovule morphology, megasporogenesis, and megagametogenesis processes were examined in Hydrocleys nymphoides , Alisma plantago-aquatica , and Sagittaria montevidensis. Each of these species belongs to a different clade within the Alismataceae family. It is worth mentioning that the genus Hydrocleys previously belonged to the Limnocharitaceae family but is now classified within the Alismataceae. Flowers in different developmental stages were processed following classical histological methods for their observation with bright-field microscope. The three species present an anatropous and bitegmic mature ovule. This is tenuinucellate in A. plantago-aquatica and S. montevidensis and pseudo-crassinucellate in H. nymphoides . Although all three species have the same type of megasporogenesis, they differ in the megagametogenesis and in the total number of nuclei and cells that form the mature gametophyte. H. nymphoides has a female gametophyte composed of four cells and four nuclei, while A. plantago-aquatica and S. montevidensis have a female gametophyte of five cells and six nuclei. The results are discussed according to the phylogenetic position of each of the species. Moreover, new types of megagametophyte development are described: Hydrocleys and Sagittaria types. The reduction of the female gametophyte with respect to the Polygonum type is found in families belonging to the ANA grade and in other aquatic families within the order Alismatales. We infer that the reduction in the number of cells and nuclei in the female gametophyte is characteristic of species that inhabit aquatic environments. Future studies in aquatic species belonging to other families would be necessary to confirm this hypothesis.