Multifunctional sprayable carboxymethyl chitosan/polyphenol hydrogel for wound healing

• Published in: International journal of biological macromolecules 2024-08, Vol.275 (Pt 1), p.133303, Article 133303
• Main Authors: Xue, Rui, He, Linyun, Wu, Jie, Kong, Xiangze, Wang, Qiuting, Chi, Yaping, Liu, Ji, Wang, Zhe, Zeng, Kewu, Chen, Wen, Ren, Huanhuan, Han, Bo
• Format: Article
• Language: English
• Subjects: angiogenesis; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Bandages; chitosan; Chitosan - analogs & derivatives; Chitosan - chemistry; Chitosan - pharmacology; collagen; Dual syringe spray hydrogel; Escherichia coli; Escherichia coli - drug effects; gelation; hydrogels; Hydrogels - chemistry; Hydrogels - pharmacology; hydrogen; Hydrogen bonding; Male; polyphenols; Rats; Staphylococcus aureus; Staphylococcus aureus - drug effects; syringes; Turkish gall extract; Wound Healing - drug effects; wound treatment
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.133303

The use of facile methods to synthesize environmentally friendly and multifunctional hydrogel dressings is still a major challenge in development. Herein, Turkish gall extract (TGE) and carboxymethyl chitosan (CMCS) were combined and sprayed using a dual syringe to form a multifunctional TGE-CMCS hydrogel (TC gel) in one step through abundant hydrogen bonding between functional groups as a green approach. TC gel showed rapid gelation at 19.0 ± 2.9 s. Apart from the advantage of being able to adapt to different wound shapes, TC gel retained the antioxidant, antibacterial, hemostatic and anti-inflammatory properties of TGE. In vitro antibacterial experiments showed that TC-gel eliminated 98.27 ± 0.79 % of Staphylococcus aureus and 98.87 ± 1.08 % of Escherichia coli. Compared with TGE or CMCS alone, TC gel accelerates skin wound healing due to its three-dimensional network structure and continuous release of active components at the wound site, enhancing re-epithelialization, improving collagen deposition, and increasing angiogenesis. The wound healing rate of full-thickness skin defect rats treated with TC gel was 93.98 ± 0.63 % on the 10th day. These results suggest that TC gel combined with a facile and scalable manufacturing method is a promising multifunctional wound dressing for clinical wound management.