Quantification, phytochemical evaluation, and identification of pomegranate yellow peel constituents using LC-MS/MS, the effect of the solvent extract on the antioxidant, in vitro anti-inflammatory, and antibacterial activities

• Published in: Industrial crops and products 2024-11, Vol.219, p.118780, Article 118780
• Main Authors: Boukezzoula, Amina, Boudemagh, Djalila, Palko, Nadezhda, Grishina, Maria, Bensouici, Chawki, Bounekhel, Mahmoud, AlShamaileh, Ehab, Dahamna, Saliha
• Format: Article
• Language: English
• Subjects: Anti-inflammatory; Antibacterial activity; antibacterial properties; Antioxidant activity; antioxidants; computer simulation; cultivars; gallic acid; metabolism; Phenolic compounds; phytochemicals; pomegranates; solvents; tannins; therapeutics; Yellow peel pomegranate
• ISSN: 0926-6690
• DOI: 10.1016/j.indcrop.2024.118780

Yellow pomegranate peel represents a promising source of bioactive compounds with potential therapeutic applications. This research focused on identifying the optimal solvent for extracting phenolic compounds from Algerian yellow pomegranate cultivars. Several antioxidant assays, including DPPH, GOR, CUPRAC, ABTS, reducing power, and phenanthroline, were employed to evaluate the extracts. Additionally, in vitro anti-inflammatory and antibacterial activities were assessed, complemented by in silico analyses. Molecular modeling explored the interaction between specific compounds (punicalagin-β, punicalagin-α, punicalin, gallic acid, and tannic acid) and various solvents. The study considered solvent effects using the MERA model and predicted biological activity and metabolism via CiS and CoMin algorithms. The aqueous extract exhibited remarkable antioxidant capacity, with notable IC50 values in ABTS (7.05±0.12 µg/mL), CUPRAC (7.40±0.79 µg/mL), reducing power (16.00±4.02 µg/mL), and phenanthroline assay (10.23±1.95 µg/mL). Furthermore, the hydro-methanolic extract displayed superior antibacterial efficacy against selected bacterial strains, while the aqueous and methanolic extracts exhibited limited activity. These findings underscore the potential of yellow pomegranate peel as a source of bioactive molecules with significant antioxidant, anti-inflammatory, and antibacterial properties. [Display omitted] •Yellow pomegranate peel exhibits significant potential as a source of bioactive compounds with potential applications in the treatment of various diseases.•The choice of solvent significantly influences the concentration of total phenolic compounds, consequently affecting the antioxidant activity of the extracts.•The aqueous extract of yellow pomegranate peels showed the highest antioxidant potency among the tested extracts.•The hydro-ethanolic extract exhibited superior efficacy against certain bacterial strains, whereas the aqueous and methanolic extracts displayed limited antibacterial activity.