The Description of Pseudoalteromonas apostichopi sp. nov., Vibrio apostichopi sp. nov., and Marinobacter apostichopi sp. nov. from the Fertilized Eggs and Larvae of Apostichopus japonicus

• Published in: Current microbiology 2024-08, Vol.81 (8), p.246-246, Article 246
• Main Authors: Kudo, Rika, Yamano, Ryota, Yu, Juanwen, Hatakeyama, Shuya, Jiang, Chunqi, Mino, Sayaka, Yamaki, Shogo, Ando, Yasuhiro, Sakai, Yuichi, Sawabe, Tomoo
• Format: Article
• Language: English
• Subjects: Amino acid sequence; Animals; Apostichopus japonicus; Bacteria; Bacterial Typing Techniques; Biomedical and Life Sciences; Biotechnology; chemotaxonomy; DNA, Bacterial - genetics; Eggs; Fatty Acids - analysis; Fatty Acids - chemistry; Genera; Genome, Bacterial; Genomic analysis; genomics; genus; Larva - microbiology; Larvae; Life Sciences; Marinobacter; Marinobacter - classification; Marinobacter - genetics; Marinobacter - isolation & purification; Microbiology; Multilocus Sequence Typing; New species; Novel Prokaryotic Taxa; Nucleotide sequence; Nucleotides; phenotype; Phylogenetics; Phylogeny; Pseudoalteromonas; Pseudoalteromonas - classification; Pseudoalteromonas - genetics; Pseudoalteromonas - isolation & purification; RNA, Ribosomal, 16S - genetics; Sequence analysis; species; Stichopus - microbiology; Strains (organisms); Vibrio; Vibrio - classification; Vibrio - genetics; Vibrio - isolation & purification; Vibrio lentus; Zygote - microbiology
• ISSN: 0343-8651; 1432-0991; 1432-0991
• DOI: 10.1007/s00284-024-03751-4

Three novel bacterial strains, FE4 T , FE10 T , and LA51 T , which are phylogenetically affiliated to the genera Pseudoalteromonas , Vibrio , or Marinobacter , respectively, isolated from fertilized eggs and juveniles of sea cucumber Apostichopus japonicus were characterized by a genome-based taxonomical approach including multilocus sequence analysis (MLSA) combined with classical phenotypic and chemotaxonomic characterizations. A molecular network reconstructed on the basis of nucleotide sequences of four phylogenetic maker protein genes revealed that the strains FE4 T , FE10 T , and LA51 T were closely related to Pseudoalteromonas shioyasakiensis , Vibrio lentus , and Marinobacter similis , respectively. Average nucleotide identity (ANI) comparisons against phylogenetically related species to FE4 T , FE10 T , and LA51 T demonstrated that each newly described strain could not be identified as any previously described species within each genus showing