Loading…
Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings
Microbially induced carbonate precipitation (MICP) immobilizes toxic metals and reduces their bioavailability in aqueous systems. However, its application in the treatment of acid mine drainage (AMD) is poorly understood. In this study, the genomes of Sporosarcina sp. UB5 and UB10 were sequenced. Ur...
Saved in:
| Published in: | Journal of hazardous materials 2024-09, Vol.476, p.135005, Article 135005 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-c276t-48fb6d44ce18c37836dcf1f0218503faf1ec725f8065ce5dbefde3c73f92a66f3 |
| container_end_page | |
| container_issue | |
| container_start_page | 135005 |
| container_title | Journal of hazardous materials |
| container_volume | 476 |
| creator | Cuaxinque-Flores, Gustavo Talavera-Mendoza, Oscar Aguirre-Noyola, José Luis Hernández-Flores, Giovanni Martínez-Miranda, Verónica Rosas-Guerrero, Víctor Martínez-Romero, Esperanza |
| description | Microbially induced carbonate precipitation (MICP) immobilizes toxic metals and reduces their bioavailability in aqueous systems. However, its application in the treatment of acid mine drainage (AMD) is poorly understood. In this study, the genomes of Sporosarcina sp. UB5 and UB10 were sequenced. Urease, carbonic anhydrases, and metal resistance genes were identified and enzymatic assays were performed for their validation. The geochemical mechanism of precipitation in AMD was elucidated through geo-mineralogical analysis. Sporosarcina sp. UB5 was shown to be a new genomospecies, with an average nucleotide identity |
| doi_str_mv | 10.1016/j.jhazmat.2024.135005 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153679904</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S030438942401584X</els_id><sourcerecordid>3079855209</sourcerecordid><originalsourceid>FETCH-LOGICAL-c276t-48fb6d44ce18c37836dcf1f0218503faf1ec725f8065ce5dbefde3c73f92a66f3</originalsourceid><addsrcrecordid>eNqFkUFv1DAQhS0EokvhJ4B85JLFjmMn4YJQBbRSEQfK2ZrY412vEjvYSaX2X_EP8WoXrj1ZY733ZvQ-Qt5ytuWMqw-H7WEPjxMs25rVzZYLyZh8Rja8a0UlhFDPyYYJ1lSi65sL8irnA2OMt7J5SS7KX69U12zIn-9xRLOOkCgES3cYzR4nb2CkA2SfaXS0jCkOHsbxgfpgV4OWGkhDDLAgnRMaP_sFFh8DdTHRJWEZwo6C8ba4A1KbwAfY4Ud6t8dizngMBhriPY705xxTzJBM0ZQTQpxinksqZupSnE4RC_ixhObX5IWDMeOb83tJfn39cnd1Xd3--HZz9fm2MnWrlqrp3KBs0xjknRFtJ5Q1jjtW804y4cBxNG0tXceUNCjtgM6iMK1wfQ1KOXFJ3p9y5xR_r5gXPflscBwhYFyzFlwK1fY9a56WsrbvpKxZX6TyJC2V5pzQ6Tn5CdKD5kwfweqDPoPVR7D6BLb43p1XrMOE9r_rH8ki-HQSYOnk3mPSuRQYCitfAC3aRv_Eir9GuLuL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3079855209</pqid></control><display><type>article</type><title>Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings</title><source>ScienceDirect Freedom Collection</source><creator>Cuaxinque-Flores, Gustavo ; Talavera-Mendoza, Oscar ; Aguirre-Noyola, José Luis ; Hernández-Flores, Giovanni ; Martínez-Miranda, Verónica ; Rosas-Guerrero, Víctor ; Martínez-Romero, Esperanza</creator><creatorcontrib>Cuaxinque-Flores, Gustavo ; Talavera-Mendoza, Oscar ; Aguirre-Noyola, José Luis ; Hernández-Flores, Giovanni ; Martínez-Miranda, Verónica ; Rosas-Guerrero, Víctor ; Martínez-Romero, Esperanza</creatorcontrib><description>Microbially induced carbonate precipitation (MICP) immobilizes toxic metals and reduces their bioavailability in aqueous systems. However, its application in the treatment of acid mine drainage (AMD) is poorly understood. In this study, the genomes of Sporosarcina sp. UB5 and UB10 were sequenced. Urease, carbonic anhydrases, and metal resistance genes were identified and enzymatic assays were performed for their validation. The geochemical mechanism of precipitation in AMD was elucidated through geo-mineralogical analysis. Sporosarcina sp. UB5 was shown to be a new genomospecies, with an average nucleotide identity < 95 % (ANI) and DNA-DNA hybridization < 70 % (DDH) whereas UB10 is close to S. pasteurii. UB5 contained two urease operons, whereas only one was identified in UB10. The ureolytic activities of UB5 and UB10 were 122.67 ± 15.74 and 131.70 ± 14.35 mM NH4+ min−1, respectively. Both strains feature several carbonic anhydrases of the α, β, or γ families, which catalyzed the precipitation of CaCO3. Only Sporosarcina sp. UB5 was able to immobilize metals and neutralize AMD. Geo-mineralogical analyses revealed that UB5 directly immobilized Fe (1–23 %), Mn (0.65–1.33 %) and Zn (0.8–3 %) in AMD via MICP and indirectly through adsorption to calcite and binding to bacterial cell walls. The MICP-treated AMD exhibited high removal rates (>67 %) for Ag, Al, As, Ca, Cd, Co, Cu, Fe, Mn, Pb, and Zn, and a removal rate of 15 % for Mg. This study provides new insights into the MICP process and its applications to AMD treatment using autochthonous strains.
[Display omitted]
•Sporosarcina sp. UB5 is a new genomospecies, whereas UB10 is close to S. pasteurii clade.•Sporosarcina sp. UB5 and UB10 have urease operon and α, β and γ carbonic anhydrases.•Urease and carbonic anhydrase induce CaCO3 precipitation.•Sporosarcina sp. UB5 neutralizes AMD and precipitates Fe, Mn and Zn as carbonates.•The cell wall of UB5 contains ligands for metal binding and mineral nucleation.</description><identifier>ISSN: 0304-3894</identifier><identifier>ISSN: 1873-3336</identifier><identifier>EISSN: 1873-3336</identifier><identifier>DOI: 10.1016/j.jhazmat.2024.135005</identifier><identifier>PMID: 38996684</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>acid mine drainage ; adsorption ; bacteria ; bioavailability ; Bioremediation ; calcite ; carbonate dehydratase ; carbonates ; Carbonic anhydrase ; Metal bio-immobilization ; metal tolerance ; nucleic acid hybridization ; operon ; Sporosarcina ; Toxic metals and metalloids ; toxicity ; Urease</subject><ispartof>Journal of hazardous materials, 2024-09, Vol.476, p.135005, Article 135005</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c276t-48fb6d44ce18c37836dcf1f0218503faf1ec725f8065ce5dbefde3c73f92a66f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38996684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cuaxinque-Flores, Gustavo</creatorcontrib><creatorcontrib>Talavera-Mendoza, Oscar</creatorcontrib><creatorcontrib>Aguirre-Noyola, José Luis</creatorcontrib><creatorcontrib>Hernández-Flores, Giovanni</creatorcontrib><creatorcontrib>Martínez-Miranda, Verónica</creatorcontrib><creatorcontrib>Rosas-Guerrero, Víctor</creatorcontrib><creatorcontrib>Martínez-Romero, Esperanza</creatorcontrib><title>Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings</title><title>Journal of hazardous materials</title><addtitle>J Hazard Mater</addtitle><description>Microbially induced carbonate precipitation (MICP) immobilizes toxic metals and reduces their bioavailability in aqueous systems. However, its application in the treatment of acid mine drainage (AMD) is poorly understood. In this study, the genomes of Sporosarcina sp. UB5 and UB10 were sequenced. Urease, carbonic anhydrases, and metal resistance genes were identified and enzymatic assays were performed for their validation. The geochemical mechanism of precipitation in AMD was elucidated through geo-mineralogical analysis. Sporosarcina sp. UB5 was shown to be a new genomospecies, with an average nucleotide identity < 95 % (ANI) and DNA-DNA hybridization < 70 % (DDH) whereas UB10 is close to S. pasteurii. UB5 contained two urease operons, whereas only one was identified in UB10. The ureolytic activities of UB5 and UB10 were 122.67 ± 15.74 and 131.70 ± 14.35 mM NH4+ min−1, respectively. Both strains feature several carbonic anhydrases of the α, β, or γ families, which catalyzed the precipitation of CaCO3. Only Sporosarcina sp. UB5 was able to immobilize metals and neutralize AMD. Geo-mineralogical analyses revealed that UB5 directly immobilized Fe (1–23 %), Mn (0.65–1.33 %) and Zn (0.8–3 %) in AMD via MICP and indirectly through adsorption to calcite and binding to bacterial cell walls. The MICP-treated AMD exhibited high removal rates (>67 %) for Ag, Al, As, Ca, Cd, Co, Cu, Fe, Mn, Pb, and Zn, and a removal rate of 15 % for Mg. This study provides new insights into the MICP process and its applications to AMD treatment using autochthonous strains.
[Display omitted]
•Sporosarcina sp. UB5 is a new genomospecies, whereas UB10 is close to S. pasteurii clade.•Sporosarcina sp. UB5 and UB10 have urease operon and α, β and γ carbonic anhydrases.•Urease and carbonic anhydrase induce CaCO3 precipitation.•Sporosarcina sp. UB5 neutralizes AMD and precipitates Fe, Mn and Zn as carbonates.•The cell wall of UB5 contains ligands for metal binding and mineral nucleation.</description><subject>acid mine drainage</subject><subject>adsorption</subject><subject>bacteria</subject><subject>bioavailability</subject><subject>Bioremediation</subject><subject>calcite</subject><subject>carbonate dehydratase</subject><subject>carbonates</subject><subject>Carbonic anhydrase</subject><subject>Metal bio-immobilization</subject><subject>metal tolerance</subject><subject>nucleic acid hybridization</subject><subject>operon</subject><subject>Sporosarcina</subject><subject>Toxic metals and metalloids</subject><subject>toxicity</subject><subject>Urease</subject><issn>0304-3894</issn><issn>1873-3336</issn><issn>1873-3336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS0EokvhJ4B85JLFjmMn4YJQBbRSEQfK2ZrY412vEjvYSaX2X_EP8WoXrj1ZY733ZvQ-Qt5ytuWMqw-H7WEPjxMs25rVzZYLyZh8Rja8a0UlhFDPyYYJ1lSi65sL8irnA2OMt7J5SS7KX69U12zIn-9xRLOOkCgES3cYzR4nb2CkA2SfaXS0jCkOHsbxgfpgV4OWGkhDDLAgnRMaP_sFFh8DdTHRJWEZwo6C8ba4A1KbwAfY4Ud6t8dizngMBhriPY705xxTzJBM0ZQTQpxinksqZupSnE4RC_ixhObX5IWDMeOb83tJfn39cnd1Xd3--HZz9fm2MnWrlqrp3KBs0xjknRFtJ5Q1jjtW804y4cBxNG0tXceUNCjtgM6iMK1wfQ1KOXFJ3p9y5xR_r5gXPflscBwhYFyzFlwK1fY9a56WsrbvpKxZX6TyJC2V5pzQ6Tn5CdKD5kwfweqDPoPVR7D6BLb43p1XrMOE9r_rH8ki-HQSYOnk3mPSuRQYCitfAC3aRv_Eir9GuLuL</recordid><startdate>20240905</startdate><enddate>20240905</enddate><creator>Cuaxinque-Flores, Gustavo</creator><creator>Talavera-Mendoza, Oscar</creator><creator>Aguirre-Noyola, José Luis</creator><creator>Hernández-Flores, Giovanni</creator><creator>Martínez-Miranda, Verónica</creator><creator>Rosas-Guerrero, Víctor</creator><creator>Martínez-Romero, Esperanza</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240905</creationdate><title>Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings</title><author>Cuaxinque-Flores, Gustavo ; Talavera-Mendoza, Oscar ; Aguirre-Noyola, José Luis ; Hernández-Flores, Giovanni ; Martínez-Miranda, Verónica ; Rosas-Guerrero, Víctor ; Martínez-Romero, Esperanza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-48fb6d44ce18c37836dcf1f0218503faf1ec725f8065ce5dbefde3c73f92a66f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>acid mine drainage</topic><topic>adsorption</topic><topic>bacteria</topic><topic>bioavailability</topic><topic>Bioremediation</topic><topic>calcite</topic><topic>carbonate dehydratase</topic><topic>carbonates</topic><topic>Carbonic anhydrase</topic><topic>Metal bio-immobilization</topic><topic>metal tolerance</topic><topic>nucleic acid hybridization</topic><topic>operon</topic><topic>Sporosarcina</topic><topic>Toxic metals and metalloids</topic><topic>toxicity</topic><topic>Urease</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cuaxinque-Flores, Gustavo</creatorcontrib><creatorcontrib>Talavera-Mendoza, Oscar</creatorcontrib><creatorcontrib>Aguirre-Noyola, José Luis</creatorcontrib><creatorcontrib>Hernández-Flores, Giovanni</creatorcontrib><creatorcontrib>Martínez-Miranda, Verónica</creatorcontrib><creatorcontrib>Rosas-Guerrero, Víctor</creatorcontrib><creatorcontrib>Martínez-Romero, Esperanza</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of hazardous materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cuaxinque-Flores, Gustavo</au><au>Talavera-Mendoza, Oscar</au><au>Aguirre-Noyola, José Luis</au><au>Hernández-Flores, Giovanni</au><au>Martínez-Miranda, Verónica</au><au>Rosas-Guerrero, Víctor</au><au>Martínez-Romero, Esperanza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings</atitle><jtitle>Journal of hazardous materials</jtitle><addtitle>J Hazard Mater</addtitle><date>2024-09-05</date><risdate>2024</risdate><volume>476</volume><spage>135005</spage><pages>135005-</pages><artnum>135005</artnum><issn>0304-3894</issn><issn>1873-3336</issn><eissn>1873-3336</eissn><abstract>Microbially induced carbonate precipitation (MICP) immobilizes toxic metals and reduces their bioavailability in aqueous systems. However, its application in the treatment of acid mine drainage (AMD) is poorly understood. In this study, the genomes of Sporosarcina sp. UB5 and UB10 were sequenced. Urease, carbonic anhydrases, and metal resistance genes were identified and enzymatic assays were performed for their validation. The geochemical mechanism of precipitation in AMD was elucidated through geo-mineralogical analysis. Sporosarcina sp. UB5 was shown to be a new genomospecies, with an average nucleotide identity < 95 % (ANI) and DNA-DNA hybridization < 70 % (DDH) whereas UB10 is close to S. pasteurii. UB5 contained two urease operons, whereas only one was identified in UB10. The ureolytic activities of UB5 and UB10 were 122.67 ± 15.74 and 131.70 ± 14.35 mM NH4+ min−1, respectively. Both strains feature several carbonic anhydrases of the α, β, or γ families, which catalyzed the precipitation of CaCO3. Only Sporosarcina sp. UB5 was able to immobilize metals and neutralize AMD. Geo-mineralogical analyses revealed that UB5 directly immobilized Fe (1–23 %), Mn (0.65–1.33 %) and Zn (0.8–3 %) in AMD via MICP and indirectly through adsorption to calcite and binding to bacterial cell walls. The MICP-treated AMD exhibited high removal rates (>67 %) for Ag, Al, As, Ca, Cd, Co, Cu, Fe, Mn, Pb, and Zn, and a removal rate of 15 % for Mg. This study provides new insights into the MICP process and its applications to AMD treatment using autochthonous strains.
[Display omitted]
•Sporosarcina sp. UB5 is a new genomospecies, whereas UB10 is close to S. pasteurii clade.•Sporosarcina sp. UB5 and UB10 have urease operon and α, β and γ carbonic anhydrases.•Urease and carbonic anhydrase induce CaCO3 precipitation.•Sporosarcina sp. UB5 neutralizes AMD and precipitates Fe, Mn and Zn as carbonates.•The cell wall of UB5 contains ligands for metal binding and mineral nucleation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38996684</pmid><doi>10.1016/j.jhazmat.2024.135005</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3894 |
| ispartof | Journal of hazardous materials, 2024-09, Vol.476, p.135005, Article 135005 |
| issn | 0304-3894 1873-3336 1873-3336 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153679904 |
| source | ScienceDirect Freedom Collection |
| subjects | acid mine drainage adsorption bacteria bioavailability Bioremediation calcite carbonate dehydratase carbonates Carbonic anhydrase Metal bio-immobilization metal tolerance nucleic acid hybridization operon Sporosarcina Toxic metals and metalloids toxicity Urease |
| title | Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T15%3A42%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20and%20geochemical%20basis%20of%20microbially%20induced%20carbonate%20precipitation%20for%20treating%20acid%20mine%20drainage:%20The%20case%20of%20a%20novel%20Sporosarcina%20genomospecies%20from%20mine%20tailings&rft.jtitle=Journal%20of%20hazardous%20materials&rft.au=Cuaxinque-Flores,%20Gustavo&rft.date=2024-09-05&rft.volume=476&rft.spage=135005&rft.pages=135005-&rft.artnum=135005&rft.issn=0304-3894&rft.eissn=1873-3336&rft_id=info:doi/10.1016/j.jhazmat.2024.135005&rft_dat=%3Cproquest_cross%3E3079855209%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c276t-48fb6d44ce18c37836dcf1f0218503faf1ec725f8065ce5dbefde3c73f92a66f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3079855209&rft_id=info:pmid/38996684&rfr_iscdi=true |