Fabrication of alginate composite hydrogel encapsulated retinoic acid and nano Se doped biphasic CaP to augment in situ mineralization and osteoimmunomodulation for bone regeneration

• Published in: International journal of biological macromolecules 2024-08, Vol.275 (Pt 1), p.133597, Article 133597
• Main Authors: Singhmar, Ritu, Son, Yumi, Jo, Yoo Jung, Zo, Sunmi, Min, Bong Ki, Sood, Ankur, Han, Sung Soo
• Format: Article
• Language: English
• Subjects: alginates; Alginates - chemistry; Animals; biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; biomineralization; bone formation; Bone Regeneration - drug effects; Bone tissue engineering; bones; calcification; Calcification, Physiologic - drug effects; calcium phosphates; Cell Line; hydrogels; Hydrogels - chemistry; Hydrogels - pharmacology; Hydroxyapatites - chemistry; Hydroxyapatites - pharmacology; Immunomodulation - drug effects; landscapes; mechanical properties; Mice; microstructure; Nanocomposites; nanoparticles; Nanoparticles - chemistry; Osteoblasts - drug effects; Osteogenesis - drug effects; Osteoimmunomodulation; retinoic acid; selenium; Selenium - chemistry; Selenium - pharmacology; Tissue Engineering - methods; tissue repair; Tissue Scaffolds - chemistry; Tretinoin - chemistry; Tretinoin - pharmacology
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.133597

Bone tissue engineering endows alternates to support bone defects/injuries that are circumscribed to undergo orchestrated process of remodeling on its own. In this regard, hydrogels have emerged as a promising platform that can confront irregular defects and encourage in situ bone repair. In this study, we aimed to develop a new approach for bone tissue regeneration by developing an alginate based composite hydrogel incorporating selenium doped biphasic calcium phosphate nanoparticles, and retinoic acid. The fabricated hydrogel was physiochemically evaluated for morphological, bonding, and mechanical behavior. Additionally, the biological response of the fabricated hydrogel was evaluated on MC3T3-E1 pre-osteoblast cells. The developed composite hydrogel confers excellent biocompatibility, and osteoconductivity owing to the presence of alginate, and biphasic calcium phosphate, while selenium presents pro osteogenic, antioxidative, and immunomodulatory properties. The hydrogels exhibited highly porous microstructure, superior mechanical attributes, with enhanced calcification, and biomineralization abilities in vitro. By combining the osteoconductive properties of biphasic calcium phosphate with multifaceted benefits of selenium and retinoic acid, the fabricated composite hydrogel offers a potential transformation in the landscape of bone defect treatment. This strategy could direct a versatile and effective approach to tackle complex bone injuries/defects and present potential for clinical translation. •The research work involves enhancement in osteoconductivity and immunomodulation in bone tissue repair•The developed composite hydrogel has high mechanical stability and enhanced calcification•This work presents a new approach to increase proliferation, differentiation and mineralization of bone cells.•The reported work investigates the synergistic effect of selenium and retinoic acid in bone tissue engineering