Preparation and evaluation of microencapsulated delivery system of recombinant interferon alpha protein from rainbow trout

• Published in: International journal of biological macromolecules 2024-07, Vol.273 (Pt 1), p.132872, Article 132872
• Main Authors: Ouyang, Ping, Li, Yankai, Wei, Wenyan, Li, Qiunan, Liu, Jiaxing, MaYang, Li, Shuhan, Zhou, Yongheng, Chen, Defang, Geng, Yi, Huang, Xiaoli
• Format: Article
• Language: English
• Subjects: Animals; antiviral agents; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; aquaculture; blood serum; Capsules; Carboxymethyl chitosan; carp; chitosan; Chitosan - analogs & derivatives; Chitosan - chemistry; Cyprinus carpio; Drug Compounding; Drug Delivery Systems; emulsifying; emulsions; Fish Diseases - drug therapy; Fish Diseases - virology; Infectious hematopoietic necrosis virus; Infectious hematopoietic necrosis virus - drug effects; Interferon; Interferon-alpha - pharmacology; interferons; Microcapsule delivery system; microencapsulation; Oncorhynchus mykiss; Oncorhynchus mykiss - virology; Recombinant Proteins - pharmacology; Resistant starch; risk; survival rate
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.132872

Diseases caused by viruses pose a significant risk to the health of aquatic animals, for which there are presently no efficacious remedies. Interferon (IFN) serving as an antiviral agent, is frequently employed in clinical settings. Due to the unique living conditions of aquatic animals, traditional injection of interferon is cumbersome, time-consuming and labor-intensive. This study aimed to prepare IFN microcapsules through emulsion technique by using resistant starch (RS) and carboxymethyl chitosan (CMCS). Optimization was achieved using the Box-Behnken design (BBD) response surface technique, followed by the creation of microcapsules through emulsification. With RS at a concentration of 1.27 %, a water‑oxygen ratio of 3.3:7.4, CaCl2 at 13.67 %, CMCS at 1.04 %, the rate of encapsulation can escalate to 80.92 %. Rainbow trout infected with Infectious hematopoietic necrosis virus (IHNV) and common carp infected with Spring vireemia (SVCV) exhibited a relative survival rate (RPS) of 65 % and 60 % after treated with IFN microcapsules, respectively. Moreover, the microcapsules effectively reduced the serum AST levels and enhanced the expression of IFNα, IRF3, ISG15, MX1, PKR and Viperin in IHNV-infected rainbow trout and SVCV-infected carp. In conclusion, this integrated IFN microcapsule showed potential as an antiviral agent for treatment of viral diseases in aquaculture.