DNA Methylation is Involved in Sex Determination in Spinach

DNA methylation plays a critical role in the modulation of gene expression. The role of DNA methylation in sex determination was investigated in spinach. The differentiated cytosine CpG methylation profiles of CCGG motifs were assessed with methylation sensitivity amplification polymorphism (MSAP) i...

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Bibliographic Details
Published in:Biochemical genetics 2024-08, Vol.62 (4), p.2455-2468
Main Authors: Jia, Keli, Duan, Jiaming, Cheng, Guangqian, Li, Heng, Li, Shufen, Hu, Miao
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
CpG Islands
Cytosine
Cytosine - metabolism
Deoxyribonucleic acid
DNA
DNA Methylation
DNA, Plant - genetics
Enzymes
Females
Flowers & plants
Fragments
Gene expression
gene expression regulation
Gene polymorphism
genome
Genomes
Human Genetics
Life sciences
Males
Medical Microbiology
Original Article
Polymorphism
Sex
Sex chromosomes
Sex determination
Sex Determination Processes
Spinach
Spinacia
Spinacia oleracea - genetics
Vegetables
Y chromosomes
Zoology
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Summary:DNA methylation plays a critical role in the modulation of gene expression. The role of DNA methylation in sex determination was investigated in spinach. The differentiated cytosine CpG methylation profiles of CCGG motifs were assessed with methylation sensitivity amplification polymorphism (MSAP) in spinach. Among 442 DNA fragments from four plants, 134 methylated fragments were found. Relative proportions of methylation sites were 28.8% in male plants and 31.8% in female plants. At the same time, cytosine methylation levels were higher in females than in males in CCGG motifs of genomes in the spinach. These findings suggest that methylation of CG islands is involved in sex determination and differentiation in spinach.
ISSN:0006-2928
1573-4927
DOI:10.1007/s10528-023-10524-4