Indigofera cryptantha-induced pigmenturia in cattle in South Africa

• Published in: Toxicon (Oxford) 2024-05, Vol.242, p.107690-107690, Article 107690
• Main Authors: Botha, Christo J., Vosser, Magdaleen, Ibrahim, Mohammed I.A., du Plessis, Elizabeth, Lensink, Antoinette V., Rudolph, Wiehan J., Invernizzi, Luke
• Format: Article
• Language: English
• Subjects: bladder; Bouteloua dactyloides; Brazil; bulls; Cattle; Cenchrus ciliaris; chemical analysis; color; cytoplasm; death; epithelium; hemoglobinuria; Indigo; Indigofera; Indigofera cryptantha; Indoxyl sulfate; ingestion; liver; oxidation; pastures; pigmentation; Pigmenturia; proteinuria; renal failure; South Africa; sulfates; UPLC-UV-qTOF/MS analysis; urinalysis; urine
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2024.107690

Two field cases of reddish-black pigmenturia occurred where cattle grazed on an established Cenchrus ciliaris (blue buffalo grass) pasture in South Africa. The pasture was noticeably invaded by Indigofera cryptantha, which was heavily grazed. Apart from the discolored urine, no other clinical abnormalities were detected. Urinalysis revealed hemoglobinuria, proteinuria and an alkaline pH. When the animals were immediately removed from the infested pasture, they made an uneventful recovery. However, a bull died when one of the herds could not be removed from the I. cryptantha-infested pasture. Macroscopically, the kidneys were dark red in color and the urinary bladder contained the dark pigmented urine. Microscopically, the renal tubules contained eosinophilic, granular pigment casts in the lumen. In addition, many renal tubular epithelial cells were attenuated with granular cytoplasm and were detached from the basement membranes. Chemical analysis was performed on dried, milled plant material and two urine samples collected during the field investigations. Qualitative UPLC-UV-qTOF/MS analysis revealed the presence of indican (indoxyl-β-glucoside) in the stems, leaves and pods of I. cryptantha and indoxyl sulfate was identified, and confirmed with an analytical standard, in the urine samples. It is proposed that following ingestion of I. cryptantha, indican will be hydrolysed in the liver to indoxyl and conjugated with sulfate. Indoxyl sulfate will then be excreted in relatively high concentrations in the urine. In the alkaline urine, two indoxyl molecules might dimerize to form leucoindigo with subsequent oxidation to indigo, thus, contributing to the dark pigmentation of the urine. It is also possible that indoxyl sulfate contributed to the renal failure and death of the bull. Although I. suffruticosa-induced hemoglobinuria has been described in Brazil, this is the first report of I. cryptantha-induced pigmenturia in cattle in South Africa. •Two outbreaks of reddish-black pigmenturia in cattle in South Africa.•Ingestion of the legume, Indigofera cryptantha.•Severe pigment nephrosis confirmed microscopically.•Qualitative analysis revealed indican in stems, leaves and pods.•Qualitative analysis confirmed indoxyl sulfate in urine samples.