24-epibrassinolide enhances drought tolerance in grapevine (Vitis vinifera L.) by regulating carbon and nitrogen metabolism

• Published in: Plant cell reports 2024-09, Vol.43 (9), p.219-219, Article 219
• Main Authors: Zeng, Guihua, Wan, Zhuowu, Xie, Rui, Lei, Bingyuan, Li, Chan, Gao, Feifei, Zhang, Zhenwen, Xi, Zhumei
• Format: Article
• Language: English
• Subjects: Ammonium; Antioxidants - metabolism; Antioxidants - pharmacology; Assimilation; beta-fructofuranosidase; Biological assimilation; Biomedical and Life Sciences; Biotechnology; Brassinosteroids; Brassinosteroids - metabolism; Brassinosteroids - pharmacology; Carbon; Carbon - metabolism; Cell Biology; Damage accumulation; Damage tolerance; Drought Resistance; drought tolerance; Free radicals; Glucosyltransferases - metabolism; Glutamate-ammonia ligase; Glutamate-Ammonia Ligase - metabolism; Glutamine; Hormones; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Invertase; Life Sciences; Lipid metabolism; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Metabolism; Nitrate reductase; Nitrate Reductase - metabolism; Nitrate reduction; Nitrogen; Nitrogen - metabolism; Original Article; Oxidative metabolism; Oxidative Stress - drug effects; Peroxidation; Photosynthesis; Photosynthesis - drug effects; Plant Biochemistry; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; proline; Reductases; Scavenging; starch; Steroid hormones; Steroids, Heterocyclic - metabolism; Steroids, Heterocyclic - pharmacology; Stress, Physiological - drug effects; Sucrose; Sucrose phosphate synthase; Sucrose synthase; Vitis - drug effects; Vitis - metabolism; Vitis - physiology; Vitis vinifera; water stress
• ISSN: 0721-7714; 1432-203X; 1432-203X
• DOI: 10.1007/s00299-024-03283-y

Key message Exogenous application of 24-epibrassinolide can alleviate oxidative damage, improve photosynthetic capacity, and regulate carbon and nitrogen assimilation, thus improving the tolerance of grapevine (Vitis vinifera L.) to drought stress. Brassinosteroids (BRs) are a group of plant steroid hormones in plants and are involved in regulating plant tolerance to drought stress. This study aimed to investigate the regulation effects of BRs on the carbon and nitrogen metabolism in grapevine under drought stress. The results indicated that drought stress led to the accumulation of superoxide radicals and hydrogen peroxide and an increase in lipid peroxidation. A reduction in oxidative damage was observed in EBR-pretreated plants, which was probably due to the improved antioxidant concentration. Moreover, exogenous EBR improved the photosynthetic capacity and sucrose phosphate synthase activity, and decreased the sucrose synthase, acid invertase, and neutral invertase, resulting in improved sucrose (190%) and starch (17%) concentrations. Furthermore, EBR pretreatment strengthened nitrate reduction and ammonium assimilation. A 57% increase in nitrate reductase activity and a 13% increase in glutamine synthetase activity were observed in EBR pretreated grapevines. Meanwhile, EBR pretreated plants accumulated a greater amount of proline, which contributed to osmotic adjustment and ROS scavenging. In summary, exogenous EBR enhanced drought tolerance in grapevines by alleviating oxidative damage and regulating carbon and nitrogen metabolism.