Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants

In this study, we evaluated the effectiveness of thermotherapy in eliminating apple rubbery wood virus 2 (ARWV-2) in six infected apple varieties collected from regions in China. These plants were subjected to high-temperature treatment, followed by excision of shoot tips, which were subsequently gr...

Full description

Saved in:
Bibliographic Details
Published in:Australasian plant pathology 2024-03, Vol.53 (2), p.151-157
Main Authors: Hu, G.-J., Dong, Y.-F., Zhang, Z.-P., Fan, X.-D., Ren, F.
Format: Article
Language:English
Subjects:
Agriculture
Apples
Biomedical and Life Sciences
China
Ecology
Entomology
excision
Fruits
Germplasm
High temperature
Life Sciences
Original Research Article
Plant Pathology
Plant Sciences
Plant virus diseases
Plant viruses
rootstocks
Stem pitting
Stems
Survival
Thermal stability
Thermotherapy
Tips
Viruses
Wood
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c303t-d5f0dbd1b384cb73a1dfb9cb9678bc7889071658b925635eec50541773ae97063
container_end_page 157
container_issue 2
container_start_page 151
container_title Australasian plant pathology
container_volume 53
creator Hu, G.-J.
Dong, Y.-F.
Zhang, Z.-P.
Fan, X.-D.
Ren, F.
description In this study, we evaluated the effectiveness of thermotherapy in eliminating apple rubbery wood virus 2 (ARWV-2) in six infected apple varieties collected from regions in China. These plants were subjected to high-temperature treatment, followed by excision of shoot tips, which were subsequently grafted onto the rootstock. All varieties exhibited high thermostability, but the survival rates (average: 30.5%) of the shoot tips varied markedly. The regenerated plants were tested using semi-nested PCR; the results showed that the ARWV-2 elimination efficiencies differed greatly among the varieties. The elimination rates were > 80% for Baxianzaofu and Ruixue and
doi_str_mv 10.1007/s13313-024-00966-8
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153728356</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2967153859</sourcerecordid><originalsourceid>FETCH-LOGICAL-c303t-d5f0dbd1b384cb73a1dfb9cb9678bc7889071658b925635eec50541773ae97063</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMouK7-AU8BL16ik6Zp06Ms6wcseNFzaNJk7dI2NWmV_nuz2wXBg5cZGJ5nZngRuqZwRwHy-0AZo4xAkhKAIsuIOEELmqaCZMCSU7QAQTlhtKDn6CKEHQBNMwYLtFtbW-vadHrCzuLhw_jW7WvZT9g6j01Tt3VXDnW3xWXfNwb7USnjJ_ztXIW_aj8GnOCyq_DBmycmYOtdezT6puyGcInObNkEc3XsS_T-uH5bPZPN69PL6mFDNAM2kIpbqFRFFROpVjkraWVVoVWR5ULpXIgCcppxoYqEZ4wboznwlOaRNEUOGVui23lv793naMIg2zpo08QnjBuDZJSzPBGM79GbP-jOjb6L38kk3oug4EWkkpnS3oXgjZW9r9vST5KC3Mcv5_hljF8e4pciSmyWQoS7rfG_q_-xfgAKN4h4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2967153859</pqid></control><display><type>article</type><title>Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants</title><source>Springer Nature</source><creator>Hu, G.-J. ; Dong, Y.-F. ; Zhang, Z.-P. ; Fan, X.-D. ; Ren, F.</creator><creatorcontrib>Hu, G.-J. ; Dong, Y.-F. ; Zhang, Z.-P. ; Fan, X.-D. ; Ren, F.</creatorcontrib><description>In this study, we evaluated the effectiveness of thermotherapy in eliminating apple rubbery wood virus 2 (ARWV-2) in six infected apple varieties collected from regions in China. These plants were subjected to high-temperature treatment, followed by excision of shoot tips, which were subsequently grafted onto the rootstock. All varieties exhibited high thermostability, but the survival rates (average: 30.5%) of the shoot tips varied markedly. The regenerated plants were tested using semi-nested PCR; the results showed that the ARWV-2 elimination efficiencies differed greatly among the varieties. The elimination rates were &gt; 80% for Baxianzaofu and Ruixue and &lt; 50% for HL, Longkou no. 3, and Yishuihong. Notably, all surviving Longkou no. 3 plants remained infected with ARWV-2. The average ARWV-2 elimination efficiency was 44.3%, closely aligning with that of the apple stem pitting virus and apple stem grooving virus. This suggests that the presence of ARWV-2 may influence the ability of certain varieties to yield virus-free germplasm. Moreover, analysis of the ARWV-2 sequences from Baxianzaofu, Ruixue, Longkou no. 3, and Yishuihong revealed that the elimination rate exhibited minimal correlation with the virus diversity. The consistency and identity of the different variants within the same isolates or among the four isolates were high. Our findings can serve as a valuable foundation for developing treatment strategies to address ARWV-2 infections.</description><identifier>ISSN: 0815-3191</identifier><identifier>EISSN: 1448-6032</identifier><identifier>DOI: 10.1007/s13313-024-00966-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Apples ; Biomedical and Life Sciences ; China ; Ecology ; Entomology ; excision ; Fruits ; Germplasm ; High temperature ; Life Sciences ; Original Research Article ; Plant Pathology ; Plant Sciences ; Plant virus diseases ; Plant viruses ; rootstocks ; Stem pitting ; Stems ; Survival ; Thermal stability ; Thermotherapy ; Tips ; Viruses ; Wood</subject><ispartof>Australasian plant pathology, 2024-03, Vol.53 (2), p.151-157</ispartof><rights>The Author(s) under exclusive licence to Australasian Plant Pathology Society Inc. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c303t-d5f0dbd1b384cb73a1dfb9cb9678bc7889071658b925635eec50541773ae97063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Hu, G.-J.</creatorcontrib><creatorcontrib>Dong, Y.-F.</creatorcontrib><creatorcontrib>Zhang, Z.-P.</creatorcontrib><creatorcontrib>Fan, X.-D.</creatorcontrib><creatorcontrib>Ren, F.</creatorcontrib><title>Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants</title><title>Australasian plant pathology</title><addtitle>Australasian Plant Pathol</addtitle><description>In this study, we evaluated the effectiveness of thermotherapy in eliminating apple rubbery wood virus 2 (ARWV-2) in six infected apple varieties collected from regions in China. These plants were subjected to high-temperature treatment, followed by excision of shoot tips, which were subsequently grafted onto the rootstock. All varieties exhibited high thermostability, but the survival rates (average: 30.5%) of the shoot tips varied markedly. The regenerated plants were tested using semi-nested PCR; the results showed that the ARWV-2 elimination efficiencies differed greatly among the varieties. The elimination rates were &gt; 80% for Baxianzaofu and Ruixue and &lt; 50% for HL, Longkou no. 3, and Yishuihong. Notably, all surviving Longkou no. 3 plants remained infected with ARWV-2. The average ARWV-2 elimination efficiency was 44.3%, closely aligning with that of the apple stem pitting virus and apple stem grooving virus. This suggests that the presence of ARWV-2 may influence the ability of certain varieties to yield virus-free germplasm. Moreover, analysis of the ARWV-2 sequences from Baxianzaofu, Ruixue, Longkou no. 3, and Yishuihong revealed that the elimination rate exhibited minimal correlation with the virus diversity. The consistency and identity of the different variants within the same isolates or among the four isolates were high. Our findings can serve as a valuable foundation for developing treatment strategies to address ARWV-2 infections.</description><subject>Agriculture</subject><subject>Apples</subject><subject>Biomedical and Life Sciences</subject><subject>China</subject><subject>Ecology</subject><subject>Entomology</subject><subject>excision</subject><subject>Fruits</subject><subject>Germplasm</subject><subject>High temperature</subject><subject>Life Sciences</subject><subject>Original Research Article</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Plant virus diseases</subject><subject>Plant viruses</subject><subject>rootstocks</subject><subject>Stem pitting</subject><subject>Stems</subject><subject>Survival</subject><subject>Thermal stability</subject><subject>Thermotherapy</subject><subject>Tips</subject><subject>Viruses</subject><subject>Wood</subject><issn>0815-3191</issn><issn>1448-6032</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouK7-AU8BL16ik6Zp06Ms6wcseNFzaNJk7dI2NWmV_nuz2wXBg5cZGJ5nZngRuqZwRwHy-0AZo4xAkhKAIsuIOEELmqaCZMCSU7QAQTlhtKDn6CKEHQBNMwYLtFtbW-vadHrCzuLhw_jW7WvZT9g6j01Tt3VXDnW3xWXfNwb7USnjJ_ztXIW_aj8GnOCyq_DBmycmYOtdezT6puyGcInObNkEc3XsS_T-uH5bPZPN69PL6mFDNAM2kIpbqFRFFROpVjkraWVVoVWR5ULpXIgCcppxoYqEZ4wboznwlOaRNEUOGVui23lv793naMIg2zpo08QnjBuDZJSzPBGM79GbP-jOjb6L38kk3oug4EWkkpnS3oXgjZW9r9vST5KC3Mcv5_hljF8e4pciSmyWQoS7rfG_q_-xfgAKN4h4</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Hu, G.-J.</creator><creator>Dong, Y.-F.</creator><creator>Zhang, Z.-P.</creator><creator>Fan, X.-D.</creator><creator>Ren, F.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240301</creationdate><title>Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants</title><author>Hu, G.-J. ; Dong, Y.-F. ; Zhang, Z.-P. ; Fan, X.-D. ; Ren, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-d5f0dbd1b384cb73a1dfb9cb9678bc7889071658b925635eec50541773ae97063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agriculture</topic><topic>Apples</topic><topic>Biomedical and Life Sciences</topic><topic>China</topic><topic>Ecology</topic><topic>Entomology</topic><topic>excision</topic><topic>Fruits</topic><topic>Germplasm</topic><topic>High temperature</topic><topic>Life Sciences</topic><topic>Original Research Article</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Plant virus diseases</topic><topic>Plant viruses</topic><topic>rootstocks</topic><topic>Stem pitting</topic><topic>Stems</topic><topic>Survival</topic><topic>Thermal stability</topic><topic>Thermotherapy</topic><topic>Tips</topic><topic>Viruses</topic><topic>Wood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, G.-J.</creatorcontrib><creatorcontrib>Dong, Y.-F.</creatorcontrib><creatorcontrib>Zhang, Z.-P.</creatorcontrib><creatorcontrib>Fan, X.-D.</creatorcontrib><creatorcontrib>Ren, F.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Australasian plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, G.-J.</au><au>Dong, Y.-F.</au><au>Zhang, Z.-P.</au><au>Fan, X.-D.</au><au>Ren, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants</atitle><jtitle>Australasian plant pathology</jtitle><stitle>Australasian Plant Pathol</stitle><date>2024-03-01</date><risdate>2024</risdate><volume>53</volume><issue>2</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>0815-3191</issn><eissn>1448-6032</eissn><abstract>In this study, we evaluated the effectiveness of thermotherapy in eliminating apple rubbery wood virus 2 (ARWV-2) in six infected apple varieties collected from regions in China. These plants were subjected to high-temperature treatment, followed by excision of shoot tips, which were subsequently grafted onto the rootstock. All varieties exhibited high thermostability, but the survival rates (average: 30.5%) of the shoot tips varied markedly. The regenerated plants were tested using semi-nested PCR; the results showed that the ARWV-2 elimination efficiencies differed greatly among the varieties. The elimination rates were &gt; 80% for Baxianzaofu and Ruixue and &lt; 50% for HL, Longkou no. 3, and Yishuihong. Notably, all surviving Longkou no. 3 plants remained infected with ARWV-2. The average ARWV-2 elimination efficiency was 44.3%, closely aligning with that of the apple stem pitting virus and apple stem grooving virus. This suggests that the presence of ARWV-2 may influence the ability of certain varieties to yield virus-free germplasm. Moreover, analysis of the ARWV-2 sequences from Baxianzaofu, Ruixue, Longkou no. 3, and Yishuihong revealed that the elimination rate exhibited minimal correlation with the virus diversity. The consistency and identity of the different variants within the same isolates or among the four isolates were high. Our findings can serve as a valuable foundation for developing treatment strategies to address ARWV-2 infections.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s13313-024-00966-8</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0815-3191
ispartof Australasian plant pathology, 2024-03, Vol.53 (2), p.151-157
issn 0815-3191
1448-6032
language eng
recordid cdi_proquest_miscellaneous_3153728356
source Springer Nature
subjects Agriculture
Apples
Biomedical and Life Sciences
China
Ecology
Entomology
excision
Fruits
Germplasm
High temperature
Life Sciences
Original Research Article
Plant Pathology
Plant Sciences
Plant virus diseases
Plant viruses
rootstocks
Stem pitting
Stems
Survival
Thermal stability
Thermotherapy
Tips
Viruses
Wood
title Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T00%3A35%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Efficiency%20of%20thermotherapy%20for%20eliminating%20apple%20rubbery%20wood%20virus%202%20and%20other%20viruses%20from%20apple%20plants&rft.jtitle=Australasian%20plant%20pathology&rft.au=Hu,%20G.-J.&rft.date=2024-03-01&rft.volume=53&rft.issue=2&rft.spage=151&rft.epage=157&rft.pages=151-157&rft.issn=0815-3191&rft.eissn=1448-6032&rft_id=info:doi/10.1007/s13313-024-00966-8&rft_dat=%3Cproquest_cross%3E2967153859%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c303t-d5f0dbd1b384cb73a1dfb9cb9678bc7889071658b925635eec50541773ae97063%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2967153859&rft_id=info:pmid/&rfr_iscdi=true