Tamoxifen induces biochemical responses in Pacific oysters Magallana gigas (Thunberg, 1793) at environmentally relevant concentrations

• Published in: Marine pollution bulletin 2024-09, Vol.206, p.116696, Article 116696
• Main Authors: Paiva, Raphaella, Ferreira, Clarissa Pellegrini, Lima, Daína, Mattos, Jacó Joaquim, Pessatti, Tomás Bohn, Tisca, Juliana Fabrício, Saldaña-Serrano, Miguel, Nogueira, Diego José, Bebianno, Maria João, Bainy, Afonso Celso Dias
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant defense; biomarkers; Biomarkers - metabolism; Bivalve; catalase; Catalase - metabolism; Crassostrea gigas; Emergent contaminant; Gills - drug effects; Gills - metabolism; Glucosephosphate Dehydrogenase - metabolism; glutathione peroxidase; Glutathione Peroxidase - metabolism; Glutathione Reductase - metabolism; glutathione transferase; Glutathione Transferase - metabolism; glutathione-disulfide reductase; Mariculture; marine pollution; NADP-glucose-6-phosphate dehydrogenase; Ostreidae - drug effects; Ostreidae - metabolism; tamoxifen; Tamoxifen - toxicity; Water Pollutants, Chemical - toxicity
• ISSN: 0025-326X; 1879-3363; 1879-3363
• DOI: 10.1016/j.marpolbul.2024.116696

The activities of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH), and glutathione-S-transferase (GST) were evaluated in the gills (GI) and digestive gland (DG) of Magallana gigas oysters exposed to tamoxifen (TAM) at environmental concentrations of 10 and 100 ng L−1 for 1 and 4 days. A higher CAT activity in the GI and DG and higher GPx activity only in the DG was observed of oysters exposed to both concentrations after 1 day. Furthermore, a significant increase in GR and G6PDH, was detected in the DG after 1 day of exposure to 10 ng L−1 and only G6PDH activity increase after 1 day of exposure to 10 ng L−1 in the GI. This suggests that the DG is a tissue more sensitive to TAM exposure and was confirmed with the individual Integrated Biomarker Response version 2 index (IBRv2i), highlighting the acute stress caused by TAM and a cellular adaptation. •Environmentally relevant concentrations of TAM cause oxidative stress in oysters.•TAM increased the activity of antioxidant enzymes after 1 day of exposure.•The digestive gland was the most responsive tissue in short-term exposure to TAM.