Early activation of hepatic stellate cells induces rapid initiation of retinyl ester breakdown while maintaining lecithin:retinol acyltransferase (LRAT) activity

• Published in: Biochimica et biophysica acta. Molecular and cell biology of lipids 2024-10, Vol.1869 (7), p.159540, Article 159540
• Main Authors: Haaker, Maya W., Goossens, Vera, Hoogland, Nina A.N., van Doorne, Hidde, Wang, Ziqiong, Jansen, Jeroen W.A., Kaloyanova, Dora V., van de Lest, Chris H.A., Houweling, Martin, Vaandrager, A. Bas, Helms, J. Bernd
• Format: Article
• Language: English
• Subjects: Acyltransferases - genetics; Acyltransferases - metabolism; Animals; cell biology; Cells, Cultured; Diterpenes - metabolism; Diterpenes - pharmacology; enzymes; fibrosis; gels; Hepatic Stellate Cells - metabolism; lipids; liver; liver diseases; LRAT; metabolism; Mice; phenotype; Phosphatidyl choline; Poly-unsaturated fatty acids; Quiescent hepatic stellate cells; Rats; Retinol; Retinyl esters; Retinyl Esters - metabolism; retinyl palmitate
• ISSN: 1388-1981; 1879-2618; 1879-2618
• DOI: 10.1016/j.bbalip.2024.159540

Lecithin:retinol acyltransferase (LRAT) is the main enzyme producing retinyl esters (REs) in quiescent hepatic stellate cells (HSCs). When cultured on stiff plastic culture plates, quiescent HSCs activate and lose their RE stores in a process similar to that in the liver following tissue damage, leading to fibrosis. Here we validated HSC cultures in soft gels to study RE metabolism in stable quiescent HSCs and investigated RE synthesis and breakdown in activating HSCs. HSCs cultured in a soft gel maintained characteristics of quiescent HSCs, including the size, amount and composition of their characteristic large lipid droplets. Quiescent gel-cultured HSCs maintained high expression levels of Lrat and a RE storing phenotype with low levels of RE breakdown. Newly formed REs are highly enriched in retinyl palmitate (RP), similar to freshly isolated quiescent HSCs, which is associated with high LRAT activity. Comparison of these quiescent gel-cultured HSCs with activated plastic-cultured HSCs showed that although during early activation the total RE levels and RP-enrichment are reduced, levels of RE formation are maintained and mediated by LRAT. Loss of REs was caused by enhanced RE breakdown in activating HSCs. Upon prolonged culturing, activated HSCs have lost their LRAT activity and produce small amounts of REs by DGAT1. This study reveals unexpected dynamics in RE metabolism during early HSC activation, which might be important in liver disease as early stages are reversible. Soft gel cultures provide a promising model to study RE metabolism in quiescent HSCs, allowing detailed molecular investigations on the mechanisms for storage and release. [Display omitted] •HSCs cultured in a soft gel maintained characteristics of quiescent HSCs.•HSCs cultured in a soft gel maintained a retinyl ester storing phenotype.•During early activation of HSCs, total retinyl ester levels are reduced.•During early activation of HSCs, LRAT-mediated retinyl ester synthesis is maintained.•During early activation of HSCs, the retinyl ester species profile changes.