NRT2.1 mediates the reciprocal regulation of nitrate and NO/SNO in seedling leaves of Fraxinus mandshurica and Betula platyphylla

• Published in: Plant physiology and biochemistry 2024-10, Vol.215, p.109024, Article 109024
• Main Authors: Wang, Bo, Li, Xiaoshuang, Han, Shuyi, Yang, Haixin, Zhan, Yaguang, Fan, Guizhi
• Format: Article
• Language: English
• Subjects: absorption; Anion Transport Proteins - genetics; Anion Transport Proteins - metabolism; Betula - genetics; Betula - metabolism; Betula pendula subsp. mandshurica; Betula platyphylla; family; Fraxinus - genetics; Fraxinus - metabolism; Fraxinus mandshurica; gene expression; Gene Expression Regulation, Plant - drug effects; Nitrate; Nitrate Transporters; nitrates; Nitrates - metabolism; Nitric oxide; Nitric Oxide - metabolism; nitrogen; nitrogen metabolism; nitroprusside; NRT2.1; Plant Leaves - genetics; Plant Leaves - metabolism; plant physiology; Plant Proteins - genetics; Plant Proteins - metabolism; S-nitrosothiol; seedlings; Seedlings - drug effects; Seedlings - genetics; Seedlings - metabolism; trees
• ISSN: 0981-9428; 1873-2690; 1873-2690
• DOI: 10.1016/j.plaphy.2024.109024

Nitric oxide (NO) and S-nitrosothiol (SNO) are signal molecules and the products of nitrogen metabolism. Nitrate (NO3−) is the main nitrogen source, and nitrate transporters (NRTs) are responsible for NO3− absorption or transport. However, the interactive effect between NO3−/NRT and NO/SNO in tree plants remains ambiguous. In the present study, 25 mmol L−1 NO3− and 1 mmol L−1 NO donor sodium nitroprusside (SNP) treatment that was conducted for 24 h enhanced NO/SNO and NO3− metabolism, whereas 2.5 mmol L−1 NO3− and 80 μmol L−1 N6022 (a compound that increases SNO content) treatment reduced them in seedling leaves of Fraxinus mandshurica and Betula platyphylla. Among the nine NRT family members examined, the gene expression level of NRT2.1 had a greater response to NO/SNO and NO3− treatment in the seedling leaves of F. mandshurica and B. platyphylla. Meanwhile, FmNRT2.1 mediated NO and SNO production in seedling leaves of F. mandshurica using Agrobacterium-mediated transient transformation. These findings shed light on the reciprocal regulation between NO3− and NO/SNO in seedlings of F. mandshurica and B. platyphylla, and NRT2.1 may act as a key regulatory hub. This study revealed the regulatory relationship between NO3− and NO/SNO in seedling leaves of Fraxinus mandshurica and Betula platyphylla. High concentrations of NO3− accelerated NO/SNO metabolism, whereas low concentrations of NO3− decelerated them. NO improved NO3− metabolism, whereas SNO reduced it. NRT2.1 mediated reciprocal regulation of nitrate and NO/SNO. [Display omitted] •The regulatory relationship between NO3− and NO/SNO in Fraxinus mandshurica and Betula platyphylla seedlings were uncovered.•High concentrations of NO3− accelerated NO/SNO metabolism, whereas low concentrations of NO3− slowed down them.•NO improved NO3− metabolism, whereas SNO reduced it.•NRT2.1 mediated reciprocal regulation of nitrate and NO/SNO.