Enhancing boreal forest resilience: A four-year impact of biochar on soil quality and fungal communities

• Published in: Microbiological research 2024-06, Vol.283, p.127696-127696, Article 127696
• Main Authors: Liao, Yang-chun-zi, Pu, Hong-xiu, Jiao, Zi-wen, Palviainen, Marjo, Zhou, Xuan, Heinonsalo, Jussi, Berninger, Frank, Pumpanen, Jukka, Köster, Kajar, Sun, Hui
• Format: Article
• Language: English
• Subjects: bioactive properties; biochar; Biochar application; boreal forests; Boreal pine forests; carbon; Charcoal; cold zones; Community function; Ecosystem; enzyme activity; enzymes; forest soils; Functional genes; fungal communities; Fungal community; genes; Mycobiome; nutrient deficiencies; pyrolysis; Resilience, Psychological; Soil - chemistry; soil amendments; soil enzymes; soil fungi; Soil Microbiology; soil properties; soil quality; species richness; Taiga; temperature
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2024.127696

Boreal forests commonly suffer from nutrient deficiency due to restricted biological activity and decomposition. Biochar has been used as a promising strategy to improve soil quality, yet its impacts on forest soil microbes, particularly in cold environment, remains poorly understood. In this study, we investigated the effects of biochar, produced at different pyrolysis temperatures (500 °C and 650 °C) and applied at different amounts (0.5 kg·m−2 and 1.0 kg·m−2), on soil property, soil enzyme activity, and fungal community dynamics in a boreal forest over a span of two to four years. Our results showed that, four-year post-application of biochar produced at 650 °C and applied at 1.0 kg·m−2, significantly increased the relative abundance of Mortierellomycota and enhanced fungal species richness, α-diversity and evenness compared to the control (CK) (P < 0.05). Notably, the abundance of Phialocephala fortinii increased with the application of biochar produced at 500 °C and applied at 0.5 kg·m−2, exhibiting a positively correlation with the carbon cycling-related enzyme β-cellobiosidase. Functionally, distinct fungal gene structures were formed between different biochar pyrolysis temperatures, and between application amounts in four-year post-biochar application (P < 0.05). Additionally, correlation analyses revealed the significance of the duration post-biochar application on the soil properties, soil extracellular enzymes, soil fungal dominant phyla, fungal community and gene structures (P < 0.01). The interaction between biochar pyrolysis temperature and application amount significantly influenced fungal α-diversity (P < 0.01). Overall, these findings provide theoretical insights and practical application for biochar as soil amendment in boreal forest ecosystems.