Fatal leptospirosis in endangered Barbary macaques (Macaca sylvanus) kept in captivity: Assessing the role of sympatric rodents

• Published in: Veterinary microbiology 2024-04, Vol.291, p.110028-110028, Article 110028
• Main Authors: Beato-Benítez, Adrián, Cano-Terriza, David, Gonzálvez, Moisés, Martínez, Remigio, Pérez-Cobo, Iratxe, Ruano, María José, Guerra, Rafael, Mozos-Mora, Elena, García-Bocanegra, Ignacio
• Format: Article
• Language: English
• Subjects: captive animals; endangered species; genes; jaundice; Leptospira; Leptospira interrogans serovar Copenhageni; leptospirosis; Macaca sylvanus; microbiology; monitoring; mortality; necrosis; nephritis; Non-human primate; PCR; public health; quantitative polymerase chain reaction; Rattus norvegicus; rodent control; Rodents; seroprevalence; serotypes; Spain; sympatry; Zoo; Zoonosis; zoos
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2024.110028

Between December 2020 and January 2021, an outbreak of acute mortality in endangered Barbary macaques (Macaca sylvanus) kept in captivity was detected in a zoo in Spain. The main findings observed in the two fatally affected animals at post-mortem evaluation were jaundice, renal tubular necrosis and interstitial nephritis. Leptospira spp. infection was confirmed by real time PCR (qPCR) in different tissues in both individuals. Analyses of secY gene from a positive individual showed 100% homology with a previously published sequence corresponding to Leptospira interrogans serovar Copenhageni. Free-living sympatric brown rats (Rattus norvegicus) from the affected zoo were also analyzed, and showed a prevalence and seroprevalence of Leptospira spp. of 18.2% (4/22; 95% CI: 2.1–34.3) and 41.9% (26/62; 95% CI: 29.7–54.2), respectively. We detected seropositive sera to five different serovars of Leptospira spp. (Copenhageni, Grippotyphosa, Pomona, Canicola and Hardjo) in the rodent population, with L. Copenhageni being the predominant one. This study describes for first time an outbreak of fatal leptospirosis in captive non-human primates in Europe. Our results show that Barbary macaques, an endangered species, are highly susceptible to Leptospira spp. infection, with sympatric wild rodents being the most likely reservoir animals involved in transmission in this outbreak. Our results suggest that rodent control could be an effective measure for minimizing exposure to Leptospira spp. in zoological collections. Given the potential implications for conservation, animal and public health, non-human primates and rodents should be included in surveillance programs for Leptospira spp. in zoos. •First outbreak of fatal Leptospira spp. infection in captive non-human primates in Europe.•The endangered Barbary macaque is highly susceptible to this zoonotic bacterium.•Sympatric free-living rodents were the most likely reservoirs involved in transmission.