Antioxidant/protective effects of carob pod (Ceratonia siliqua L.) water extract against deltamethrin-induced oxidative stress/toxicity in zebrafish larvae

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2023-05, Vol.267, p.109584-109584, Article 109584
• Main Authors: Ünal, Elif, Sulukan, Ekrem, Şenol, Onur, Baran, Alper, Nadaroğlu, Hayrunnisa, Kankaynar, Meryem, Kızıltan, Tuba, Ceyhun, Saltuk Buğrahan
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; antioxidant activity; antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; apoptosis; body length; carob; Ceratonia siliqua; Danio rerio; Deltamethrin; eyes; Fabaceae - metabolism; Larva; locomotion; Metabolomics; Oxidative Stress; oxygen; scotophase; survival rate; toxicity; Zebrafish - metabolism
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2023.109584

In our study, the antioxidant capacity of carob pods water extract (CPWE) against deltamethrin (DM)-induced oxidative stress, a widely used pesticide around the world, was investigated in vitro and in vivo in a zebrafish model. The in vitro antioxidant capacity of the obtained extract was evaluated with different methods using trolox, BHA and BHT standard antioxidants. For in vivo experiments, 4hpf zebrafish embryos were exposed to 10 ppb and 25 ppb DM for 120 h and the larvae were treated with 1–10 and 100 ppm CPWE for 4 h at 72th hours. According to the results obtained, it has been determined that the exposure of zebrafish to DM during the developmental period causes important body malformations, decrease in survival rate, reduction in eye size, shortening in body length and decrease in locomotor activity in the dark period. In addition, according to the results of whole-mount staining, it was determined that DM caused a significant increase in the amount of free oxygen radicals and apoptotic cells. It was also confirmed by metabolome analysis that CPWE application for 4 h reduced DM-induced toxicity and oxidative stress. As a result, it can be said that CPWE has an important antioxidant capacity in eliminating DM-induced oxidative stress. [Display omitted] •DM causes oxidative stress in zebrafish.•CPWE has an antioxidant capacity against DM-induced oxidative stress.•CPWE affects important antioxidant metabolites and related pathways.