Combined analysis of Polygonum cuspidatum transcriptome and metabolome revealed that PcMYB62, a transcription factor, responds to methyl jasmonate and inhibits resveratrol biosynthesis

• Published in: International journal of biological macromolecules 2024-06, Vol.270 (Pt 2), p.132450, Article 132450
• Main Authors: Lin, Fan, Chen, Jianhui, Wang, Xiaowei, Ma, Hongping, Liang, Shuang, Hu, Hongyan, Fan, Haili, Wu, Zhijun, Chai, Tuanyao, Wang, Hong
• Format: Article
• Language: English
• Subjects: Acetates - metabolism; Acetates - pharmacology; Acyltransferases - genetics; Acyltransferases - metabolism; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; biosynthesis; Cyclopentanes - metabolism; Cyclopentanes - pharmacology; Fallopia japonica - genetics; Fallopia japonica - metabolism; flavonoids; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant - drug effects; genetically modified organisms; MeJA; metabolites; Metabolome; Metabolome - drug effects; metabolomics; methyl jasmonate; Oxylipins - metabolism; Oxylipins - pharmacology; PcMYB62; Plant Leaves - drug effects; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Polygonum cuspidatum; promoter regions; Resveratrol; Resveratrol - metabolism; Resveratrol - pharmacology; Reynoutria japonica; transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptome; Transcriptome - drug effects; transcriptomics; trihydroxystilbene synthase; unigenes
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.132450

A comparative transcriptomic and metabolomic analysis of Polygonum cuspidatum leaves treated with MeJA was carried out to investigate the regulatory mechanisms of its active compounds. A total of 692 metabolites and 77,198 unigenes were obtained, including 200 differentially accumulated metabolites and 6819 differentially expressed genes. We screened potential regulatory transcription factors involved in resveratrol and flavonoids biosynthesis, and successfully identified an MYB transcription factor, PcMYB62, which could significantly decrease the resveratrol content in P. cuspidatum leaves when over-expressed. PcMYB62 could directly bind to the MBS motifs in the promoter region of stilbene synthase (PcSTS) gene and repress its expression. Besides, PcMYB62 could also repress PcSTS expression and resveratrol biosynthesis in transgenic Arabidopsis thaliana. Our results provide abundant candidate genes for further investigation, and the new finding of the inhibitory role of PcMYB62 on the resveratrol biosynthesis could also potentially be used in metabolic engineering of resveratrol in P. cuspidatum.