A Peptide Microarray Platform Approach for Discovery of Immunodominant Antibody Epitopes

Immunodominant epitope discovery platforms play an important role in identifying novel biomarkers for effective immunotherapies and diagnostics. Methods to analyze the B-cell repertoire have been improved both experimentally and computationally. We developed an enhanced peptide microarray platform t...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2024-09, Vol.96 (36), p.14524-14530
Main Authors: Stafford, Phillip, Sherma, Nisha D., Peterson, Milene, Diehnelt, Chris W.
Format: Article
Language:English
Subjects:
analytical chemistry
antibodies
Antibodies, Viral - immunology
B-lymphocytes
Biomarkers
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 - virology
diagnostic techniques
Epitopes
Humans
immunodominant epitopes
Immunodominant Epitopes - chemistry
Immunodominant Epitopes - immunology
Immunoglobulin G
Immunoglobulin G - immunology
Immunotherapy
Lymphocytes B
Parameter identification
peptide microarrays
Peptides
Peptides - chemistry
Peptides - immunology
Protein Array Analysis - methods
SARS-CoV-2 - immunology
Severe acute respiratory syndrome coronavirus 2
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Summary:Immunodominant epitope discovery platforms play an important role in identifying novel biomarkers for effective immunotherapies and diagnostics. Methods to analyze the B-cell repertoire have been improved both experimentally and computationally. We developed an enhanced peptide microarray platform to discover and subsequently screen immunodominant epitopes. We utilized SARS-Cov-2 IgG positive and negative samples as a proof-of-concept to demonstrate the power of these improved peptide microarrays. The method identified significantly discriminant epitopes that classify positive and negative samples with good performance both as single peptides and in combination. We provide the assay conditions and parameters that justify the use of peptide microarrays in the selection of high-affinity epitopes, and we directly compare peptide performance against proteins. The results suggest that this platform can be used to confidently identify immunodominant antiviral epitopes while also serving as a useful tool for high-volume screening.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.4c02806