Platelet rich fibrin and simvastatin-loaded pectin-based 3D printed-electrospun bilayer scaffold for skin tissue regeneration

• Published in: International journal of biological macromolecules 2024-04, Vol.265 (Pt 1), p.130954-130954, Article 130954
• Main Authors: Tavakoli, Mohamadreza, Al-Musawi, Mastafa H., Kalali, Alma, Shekarchizadeh, Afrooz, Kaviani, Yeganeh, Mansouri, Agrin, Nasiri-Harchegani, Sepideh, Kharazi, Anousheh Zargar, Sharifianjazi, Fariborz, Sattar, Mamoona, Varshosaz, Jaleh, Mehrjoo, Morteza, Najafinezhad, Aliakbar, Mirhaj, Marjan
• Format: Article
• Language: English
• Subjects: angiogenesis; biodegradability; calcium; cell viability; chelation; collagen; crosslinking; Electrospinning; fibrin; hydrogels; modulus of elasticity; nanofibers; Pectin; pectins; Platelet-rich fibrin; polyacrylic acid; Simvastatin; Three-dimensional printing; tissue repair
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.130954

Designing multifunctional wound dressings is a prerequisite to prevent infection and stimulate healing. In this study, a bilayer scaffold (BS) with a top layer (TL) comprising 3D printed pectin/polyacrylic acid/platelet rich fibrin hydrogel (Pec/PAA/PRF) and a bottom nanofibrous layer (NL) containing Pec/PAA/simvastatin (SIM) was produced. The biodegradable and biocompatible polymers Pec and PAA were cross-linked to form hydrogels via Ca2+ activation through galacturonate linkage and chelation, respectively. PRF as an autologous growth factor (GF) source and SIM together augmented angiogenesis and neovascularization. Because of 3D printing, the BS possessed a uniform distribution of PRF in TL and an average fiber diameter of 96.71 ± 18.14 nm was obtained in NL. The Young's modulus of BS was recorded as 6.02 ± 0.31 MPa and its elongation at break was measured as 30.16 ± 2.70 %. The wound dressing gradually released growth factors over 7 days of investigation. Furthermore, the BS significantly outperformed other groups in increasing cell viability and in vivo wound closure rate (95.80 ± 3.47 % after 14 days). Wounds covered with BS healed faster with more collagen deposition and re-epithelialization. The results demonstrate that the BS can be a potential remedy for skin tissue regeneration. Graphical organizer to illustrate PRF extraction, bilayer scaffold (BS) fabrication, and in vivo wound healing promotion. [Display omitted]