A novel ultrasensitive and fast aptamer biosensor of SEB based on AuNPs-assisted metal-enhanced fluorescence

A fluorescent biosensor strategy was developed in combination with immunomagnetic separation for rapid and sensitive detection of staphylococcal enterotoxin B (SEB). Magnetic nanoparticles (MNPs) modified with aptamer of SEB could capture the SEB. Then the gold nanoparticles (AuNPs) fluorescent prob...

Full description

Saved in:
Bibliographic Details
Published in:The Science of the total environment 2023-02, Vol.858, p.159977, Article 159977
Main Authors: Chang, Xueyu, Cheng, Yaqian, Wang, Xinke, Wang, Yonghui, Liu, Xueli, Han, Tie, Gao, Zhixian, Zhou, Huanying
Format: Article
Language:English
Subjects:
Aptamer
Biosensor
biosensors
detection limit
enterotoxins
environment
Fluorescence
fluorescent dyes
immunomagnetic separation
magnetic fields
Magnetic nanoparticles
milk
nanogold
nanoparticles
oligonucleotides
Staphylococcal enterotoxin B
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A fluorescent biosensor strategy was developed in combination with immunomagnetic separation for rapid and sensitive detection of staphylococcal enterotoxin B (SEB). Magnetic nanoparticles (MNPs) modified with aptamer of SEB could capture the SEB. Then the gold nanoparticles (AuNPs) fluorescent probe was added and a “sandwich structure” was formed between AuNPs, SEB and MNPs. The MNPs-SEB-AuNPs structure could be separated with an additional magnetic field, which resulted the lower signals of AuNPs fluorescent probe. In optimal conditions, the current method displayed a broad quantitative range from 100 to 107 fg/mL and the limit of detection was 3.43 fg/mL. The recovery of SEB-spiked milk samples ranged from 92.00 to 119.00 %, which revealed that the developed method had great accuracy. Furthermore, the method was fast and economical for ultrasensitive detection. Therefore, the fluorescent biosensor based on MNPs-AuNPs is promising for the detection of other environmental and food pollutants. The sensing mechanism of the proposed MNPs-AuNPs switch for SEB detection. [Display omitted] •An ultrasensitive and fast aptamer biosensor for staphylococcal enterotoxin B (SEB) was proposed.•The MNPs-DNA conjugate was used as specific recognition and fast separate element.•The ratio of ssDNA modified AuNPs was designed for signal enhancement without additional amplification.•The strategy could be used to detect other analytes by changing the aptamer and corresponding DNA sequences.
ISSN:0048-9697
DOI:10.1016/j.scitotenv.2022.159977